Platelet-rich fibrin prepared from stored whole-blood samples.
Platelet-rich fibrin prepared from stored whole-blood samples.
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DOI：
10.1186/s40729-017-0068-4
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发表时间：
2017-12
影响因子：
2.7
通讯作者：
Kawase T
中科院分区：
文献类型：
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作者：
Isobe K;Suzuki M;Watanabe T;Kitamura Y;Suzuki T;Kawabata H;Nakamura M;Okudera T;Okudera H;Uematsu K;Nakata K;Tanaka T;Kawase T
在再生治疗中，自凝血小板浓缩物，如富血小板纤维蛋白（PRF），通常在现场制备并立即用于治疗。如果血液样本或制备的凝块可以保存数天，其临床适用性将扩大。在这里，我们从储存的全血样本中制备了PRF，并检查了它们的特性。从不吸烟的健康男性供体（年龄27 - 67岁，N = 6）收集血液样品，并立即或在储存1 - 2天后制备PRF凝块。用扫描电镜观察纤维形态。通过涉及人骨膜细胞的生物测定系统评价生物活性，而PDGF-BB浓度通过酶联免疫吸附试验测定。添加最佳量的10%CaCl2溶液可恢复含有抗凝剂（酸性柠檬酸盐葡萄糖）并在环境温度下储存长达2天的全血样本的凝血能力。在从储存的全血样本制备的PRF凝块中，纤维蛋白纤维的厚度和交联与新鲜制备的PRF凝块几乎相同。储存的全血样本中PRF提取物中的PDGF-BB浓度显著低于新鲜样本;然而，两种提取物对骨膜细胞增殖具有相似的刺激作用。从储存的全血样品制备的PRF凝块的质量没有显著降低，并且可以确保用于再生治疗。因此，所提出的方法能够实现更灵活的治疗方案，并在治疗前立即选择更合适的血小板浓缩物，而不是在采血后。
In regenerative therapy, self-clotted platelet concentrates, such as platelet-rich fibrin (PRF), are generally prepared on-site and are immediately used for treatment. If blood samples or prepared clots can be preserved for several days, their clinical applicability will expand. Here, we prepared PRF from stored whole-blood samples and examined their characteristics. Blood samples were collected from non-smoking, healthy male donors (aged 27–67 years, N = 6), and PRF clots were prepared immediately or after storage for 1–2 days. Fibrin fiber was examined by scanning electron microscopy. Bioactivity was evaluated by means of a bioassay system involving human periosteal cells, whereas PDGF-BB concentrations were determined by an enzyme-linked immunosorbent assay. Addition of optimal amounts of a 10% CaCl2 solution restored the coagulative ability of whole-blood samples that contained an anticoagulant (acid citrate dextrose) and were stored for up to 2 days at ambient temperature. In PRF clots prepared from the stored whole-blood samples, the thickness and cross-links of fibrin fibers were almost identical to those of freshly prepared PRF clots. PDGF-BB concentrations in the PRF extract were significantly lower in stored whole-blood samples than in fresh samples; however, both extracts had similar stimulatory effects on periosteal-cell proliferation. Quality of PRF clots prepared from stored whole-blood samples is not reduced significantly and can be ensured for use in regenerative therapy. Therefore, the proposed method enables a more flexible treatment schedule and choice of a more suitable platelet concentrate immediately before treatment, not after blood collection.