Immunomodulatory activities of proteins from Astragalus membranaceus waste
Immunomodulatory activities of proteins from Astragalus membranaceus waste
复制标题
DOI：
10.1002/jsfa.9650
复制
发表时间：
2019-06-01
影响因子：
4.1
通讯作者：
Chen, Gui-tang
中科院分区：
文献类型：
--
作者：
Huang, Hao;Luo, Shuang-hui;Chen, Gui-tang
背景黄芪是一种具有悠久医学应用历史的传统中药。因此，研究A.黄芪废料的开发利用，提高资源利用率。结果从A.膜壳菌废料该蛋白经DEAE-cellulose-52柱层析和Sephadex G-200分子排阻层析得到3个组分，分别命名为AMWPDG 2、AMWPDG 4和AMWPDG 6。然后，通过细胞模型实验评价它们的免疫调节活性。结果表明，该蛋白组分能显著促进脾淋巴细胞、腹腔巨噬细胞和骨髓源性细胞的增殖。AMWPDG 2表现出最高的免疫活性。AMWPDG 2、AMWPDG 4和AMWPDG 6不仅显著提高了腹腔巨噬细胞的吞噬功能和免疫调节因子（白细胞介素（IL）-6、肿瘤坏死因子-α、一氧化氮、过氧化氢）的分泌，而且促进了BMDCs中炎性细胞因子（IL-6、IL-12 p40、IL-1 β、IL-1 α）和趋化因子（CXCL 1、CCL 3）的表达。结论综合以上结果，可以认为A.膜壳菌废弃物可能是一种潜在的天然免疫调节剂。为进一步研究AMWPDG 2、AMWPDG 4和AMWPDG 6提高免疫应答的机制提供了理论依据。(c)2019化学工业协会
BACKGROUND Astragalus membranaceus is a traditional Chinese medicine that has a long history of medical applications. It is of interest to investigate the functional components of A. membranaceus waste with regard to its development and utilization and increasing resource utilization. RESULTS The protein AMWP was isolated from the A. membranaceus waste. This protein was further purified by DEAE-cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography to obtain three fractions, named AMWPDG2, AMWPDG4 and AMWPDG6. Then, their immunomodulatory activities were evaluated by using cell model experiments. The results indicated that the protein fractions could significantly increase the proliferation of splenic lymphocytes, peritoneal macrophages and bone-marrow-derived cells (BMDCs). AMWPDG2 showed the highest immunocompetence. AMWPDG2, AMWPDG4 and AMWPDG6 not only significantly improved the phagocytosis and immunomodulatory factors (interleukin (IL)-6, tumor necrosis factor-alpha, nitric oxide, hydrogen peroxide) secretion of peritoneal macrophages, but also promoted the expression of inflammatory cytokines (IL-6, IL-12 p40, IL-1 beta, IL-1 alpha) and chemokines (CXCL1, CCL3) in BMDCs. CONCLUSION Taken together, these results indicated that three protein fractions from the A. membranaceus waste might be a potential natural immunomodulator. Moreover, it also provided the theoretical basis for further researching the mechanism of AMWPDG2, AMWPDG4 and AMWPDG6 on improving the immune response. (c) 2019 Society of Chemical Industry