The cellulose synthase (CESA) gene superfamily of the moss Physcomitrella patens
The cellulose synthase (CESA) gene superfamily of the moss Physcomitrella patens
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DOI：
10.1007/s11103-006-9083-1
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发表时间：
2006-09
影响因子：
5.1
通讯作者：
A. Roberts;J. Bushoven
中科院分区：
文献类型：
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作者：
A. Roberts;J. Bushoven
拟南芥和其他种子植物的CESA基因超家族包括编码纤维素合成酶催化亚基的CESA家族和功能大部分未知的CESA样（CSL）基因的八个家族。已提出CSL基因编码合成非纤维素细胞壁多糖的进行性β-糖基转移酶。BLAST检索藓类小立碗藓（Physcomitrella patens（Hedw.）B.S. G用于鉴定与维管植物CESA、CSLA、CSLCs和CSLDs具有高度相似性的基因。然而，使用拟南芥CSLBs、CSLes和CSLGs或水稻CSLFs或CSLHs作为查询的搜索在P中没有发现额外的CESA超家族成员。patens，表明这种苔藓缺乏这些家庭的代表。内含子插入位点在拟南芥和P.在所有四个共有的基因家族中获得专利。然而，系统发育分析强烈支持独立的多样化，在苔藓和维管植物的共享家庭。由于缺乏维管植物CESAs的直系同源物，patensgenome的研究表明，苔藓和维管植物的分歧早于CESA的分歧和专业化的初生和次生细胞壁合成和不同的作用，在莲座状末端复合体。与拟南芥相反，CSLD家族是高度代表性的CSNP。专利EST。这与CSLDs在尖端生长中的功能以及尖端生长在苔藓原丝体发育中的中心作用是一致的。
TheCESAgene superfamily of Arabidopsis and other seed plants comprises theCESAfamily, which encodes the catalytic subunits of cellulose synthase, and eight families ofCESA-like(CSL) genes whose functions are largely unknown. TheCSLgenes have been proposed to encode processive β-glycosyl transferases that synthesize noncellulosic cell wall polysaccharides. BLAST searches of EST and shotgun genomic sequences from the mossPhyscomitrella patens(Hedw.) B.S.G. were used to identify genes with high similarity to vascular plantCESAs,CSLAs,CSLCs, andCSLDs. However, searches using ArabidopsisCSLBs,CSLEs, andCSLGs or riceCSLFs orCSLHs as queries identified no additionalCESAsuperfamily members inP. patens, indicating that this moss lacks representatives of these families. Intron insertion sites are highly conserved between Arabidopsis andP. patensin all four shared gene families. However, phylogenetic analysis strongly supports independent diversification of the shared families in mosses and vascular plants. The lack of orthologs of vascular plantCESAs in theP. patensgenome indicates that the divergence of mosses and vascular plants predated divergence and specialization ofCESAs for primary and secondary cell wall syntheses and for distinct roles within the rosette terminal complexes. In contrast to Arabidopsis, theCSLDfamily is highly represented amongP. patensESTs. This is consistent with the proposed function ofCSLDs in tip growth and the central role of tip growth in the development of the moss protonema.