Cellular localization of mRNAs for prostaglandin E receptor subtypes in mouse gastrointestinal tract
Cellular localization of mRNAs for prostaglandin E receptor subtypes in mouse gastrointestinal tract
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DOI：
10.1152/ajpgi.1997.272.3.g681
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发表时间：
1997-03-01
影响因子：
4.5
通讯作者：
Ichikawa, A
中科院分区：
文献类型：
--
作者：
Morimoto, K;Sugimoto, Y;Ichikawa, A
采用原位杂交技术研究了前列腺素E （PGE）受体亚型mrna在小鼠胃肠道中的区域和细胞分布。EP(1)转录本的强烈信号在粘膜肌层细胞中检测到，特别是在胃体中。EP(3)转录本的强烈信号在整个神经束的肌肠神经节神经元中被检测到。除胃表面黏液细胞外，基底腺上皮细胞也有中度EP(3) mRNA表达。EP(4) mRNA在大鼠体表上皮细胞和从体表到胃窦底部的腺体中均有中等表达。十二指肠、空肠和回肠上皮可见强EP(4)信号。在回肠中，仅在绒毛上部观察到信号。然而，未检测到EP(2)转录本的信号或微弱信号。这些发现表明PGE(2)通过至少三种不同的PGE受体调节各种胃或肠功能。
Regional and cellular distribution of mRNAs for prostaglandin E (PGE) receptor subtypes was investigated in the mouse gastrointestinal tract by in situ hybridization. Strong signals for EP(1) transcripts were detected in cells of the muscularis mucosae layer, especially in the body of the stomach. Intense signals for EP(3) transcripts were detected in neurons of the myenteric ganglia throughout the tract. Moderate EP(3) mRNA expression was also observed in fundic gland epithelial cells, except for surface mucous cells in the stomach. Expression of EP(4) mRNA was moderate in surface epithelial cells of the corpus and in glands from the surface to the base of the antrum. Strong EP(4) signals were observed in the epithelium in the duodenum, jejunum, and ileum. In the ileum, signals were only observed in the upper part of the villi. However, no or weak signals for EP(2) transcripts were detected. These findings suggest that PGE(2) modulates various gastric or intestinal functions via at least three different PGE receptors.