Binding of triethyltin to cat haemoglobin and modification of the binding sites by diethyl pyrocarbonate.
Binding of triethyltin to cat haemoglobin and modification of the binding sites by diethyl pyrocarbonate.
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三乙基锡与猫血红蛋白的结合以及焦碳酸二乙酯对结合位点的修饰。
DOI：
10.1042/bj1630583
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
W. Aldridge
中科院分区：
文献类型：
--
作者：
B. M. Elliott;W. Aldridge
猫血红蛋白结合2摩尔三乙基锡/摩尔血红蛋白。在pH6.0下用焦碳酸二乙酯对血红蛋白进行预处理，可以防止血红蛋白仅与一个位点结合，而用亚甲蓝进行光氧化则可以去除两个位点。用焦碳酸二乙酯预处理大鼠血红蛋白也会导致一个结合位点的丢失。的可能性进行了讨论，猫和大鼠血红蛋白上的两个结合位点的三乙基锡有不同的化学性质。
Cat haemoglobin binds 2 mol of triethyltin/mol of haemoglobin. Pretreatment of the haemoglobin with diethyl pyrocarbonate at pH6.0 prevents binding to one site only, whereas photo-oxidation with Methylene Blue removes both sites. Pretreatment of rat haemoglobin with diethyl pyrocarbonate also leads to the loss of one binding site. The possibility is discussed that the two binding sites for triethyltin on both cat and rat haemoglobin have a different chemical nature.