A study of the Effect of Proinflammatory Cytokines on the Epithelial Cells of Smokers, with or without COPD
A study of the Effect of Proinflammatory Cytokines on the Epithelial Cells of Smokers, with or without COPD
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DOI：
10.1016/j.arbres.2011.04.007
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发表时间：
2011-09-01
影响因子：
8
通讯作者：
Milara Paya, Javier
中科院分区：
文献类型：
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作者：
De Diego Damia, Alfredo;Cortijo Gimeno, Julio;Milara Paya, Javier
简介：香烟烟雾是COPD炎症的主要原因。区分吸烟者发展COPD的机制是多种多样的。在这项研究中，我们分析了吸烟者的呼吸道分泌物中存在的细胞因子与或没有COPD和分泌特性的分化支气管上皮细胞从个人本身暴露于烟草smoke.Material和方法：27吸烟者进行了研究，其中12人有COPD，以前没有用类固醇治疗。其中11例通过诱导痰采集标本，其余标本通过支气管镜检查后的支气管抽吸采集。IL-8的浓度。测定上清液中的IL-13和TNF α。所获得的结果进行了比较，个人与非COPD，我们研究了他们的关系与COPD的严重程度表示的程度阻塞，呼吸困难，存在高分泌和吸烟强度。采用气液界面法对4例吸烟者进行支气管上皮细胞培养。将样本暴露于增加浓度的香烟烟雾（5-20%），并测定上皮MUC 5AC、IL 8和TNF α的mRNA表达。IL-8水平与梗阻程度（r = 0.6; p < 0.05）、呼吸困难（r = 0.45; p < 0.05）及有无高分泌有关。细胞因子与吸烟习惯的强度或持续时间之间没有关系。香烟烟雾在Muc 5AC、IL 8和TNF α的RNAm表达中产生剂量依赖性增加。结论：吸烟者和患有COPD的吸烟者之间的细胞因子产生（基本上是IL 8）存在差异，这可以通过香烟烟雾对上皮细胞的直接作用来解释（C）2011 SEPAR。出版社：Elsevier Espana，S.L. All rights reserved.
Introduction: Cigarette smoke is the main cause of inflammation in COPD. The mechanisms that differentiate smokers who develop COPD are diverse. In this study, we analyzed the presence of cytokines in the respiratory secretions of smokers with or without COPD and the secretory properties of the differentiated bronchial epithelium obtained from the individuals themselves after exposure to tobacco smoke.Material and methods: Twenty-seven smokers were studied, 12 of whom had COPD that had not been previously treated with steroids. In 11, samples were obtained by means of induced sputum, and the remaining samples were collected from bronchial aspiration after bronchoscopy. Concentrations of IL-8. IL-13 and TNF alpha in the supernatant were determined. The results obtained were compared between individuals with and without COPD, and we studied their relationship with the severity of COPD as expressed by the degree of obstruction, dyspnea, presence of hypersecretion and intensity of smoking. Bronchial epithelial cell cultures were obtained by air-liquid interface in 4 smokers. The samples were exposed to increasing concentrations of cigarette smoke (5-20%) and the epithelial mRNA expressions of MUC5AC, IL8 and TNF alpha were determined.Results: COPD patients had significantly higher values of IL-8 than healthy smokers (41[22] vs. 21[12] pM). The values of IL-8 correlated significantly with the severity of the obstruction (r = 0.6; p < 0.05), dyspnea (r = 0.45; p < 0.05) and the presence of hypersecretion. There was no relationship between cytokines and the intensity or duration of the tobacco habit. Cigarette smoke produced a dose-dependent increase in the expression of RNAm for Muc5AC, IL8 and TNF alpha.Conclusions: There are differences in cytokine production (fundamentally IL8) between smokers and smokers with COPD which could be explained by the direct action of cigarette smoke on epithelial cells (C) 2011 SEPAR. Published by Elsevier Espana, S.L. All rights reserved.