Development of a cell culture system loading cyclic mechanical strain to chondrogenic cells
Development of a cell culture system loading cyclic mechanical strain to chondrogenic cells
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DOI：
10.1016/j.jbiotec.2007.08.007
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发表时间：
2008-01-20
影响因子：
4.1
通讯作者：
Suzuki, Osamu
中科院分区：
文献类型：
--
作者：
Masuda, Taisuke;Takahashi, Ichiro;Suzuki, Osamu
机械刺激被认为是调节骨骼组织细胞代谢、增殖、存活和分化的主要表观遗传因素之一。一般认为，细胞骨架可以响应于机械刺激（例如拉伸应变或流体流动）而经历重构。机械诱导的细胞变形是软骨细胞感知和响应其机械环境变化的可能的机械转导途径之一。机械应变对骨骼组织中的软骨细胞、成骨细胞和成纤维细胞等细胞的结构和功能有多种影响。然而，很少有人知道的质量和数量的机械应变和机械负荷的时间对这些细胞的分化的影响。本研究旨在探讨软骨细胞的变形的影响，并使用一种新开发的培养装置的周期性压缩，通过分析力学生物学反应的分化软骨细胞。通过以质量模式接种在PDMS膜上，在23 μ Hz下将0 - 22%应变的循环压缩加载到软骨形成细胞系ATDC 5上，假设循环变形以相同频率从膜直接转移到细胞。基于有限元建模（FEM）的分析，表征了膜内引起的压缩应变。结果表明，张应变抑制ATDC 5细胞的成软骨分化，而压应变促进ATDC 5细胞的成软骨分化，提示成软骨细胞的分化可以通过应变量和应变方式来控制。总之，我们已经开发了一个独特的应变加载培养系统，以分析各种类型的机械刺激对各种细胞活动的影响。(c)2007 Elsevier B. V.保留所有权利。
Mechanical stimulationis considered to be one of the major epigenetic factors regulating themetabolism, proliferation, survival and differentiation of cells in the skeletal tissues. It is generally accepted that the cytoskeleton can undergo remodeling in response to mechanical stimuli such as tensile strain or fluid flow. Mechanically induced cell deformation is one of the possible mechanotransduction pathways by which chondrocytes sense and respond to changes in their mechanical environment. Mechanical strain has a variety of effects on the structure and function of their cells in the skeletal tissues, such as chondrocytes, osteoblasts and fibroblasts. However, little is known about the effect of the quality and quantity of mechanical strain and the timing of mechanical loading on the differentiation of these cells. The present study was designed to investigate the effect of the deformation of chondrogenic cells, and cyclic compression using a newly developed culture device, by analyzing mechanobiological response to the differentiating chondrocytes. Cyclic compression between 0 and 22% strains, at 23 mu Hz was loaded on chondrogenic cell line ATDC5 by seeding in a mass mode on PDMS membrane, assuming direct transfer of cyclic deformation from the membrane to the cells at the same frequency. The compressive strain, induced within the membrane, was characterized based on the analysis of the finite element modeling (FEM). The results showed that the tensile strain inhibits the chondrogenic differentiation of ATDC5 cells, whereas the compressive strain enhances the chondrogenic differentiation, suggesting that the differentiation of the chondrogenic cells could be controlled by the amount and the mode of strain. In conclusion, we have developed a unique strain loading culture system to analyze the effect of various types of mechanical stimulation on various cellular activities. (c) 2007 Elsevier B.V. All rights reserved.