NONINVASIVE MEASUREMENT OF PYRUVATE AND GLUCOSE-UPTAKE AND LACTATE PRODUCTION BY SINGLE HUMAN PREIMPLANTATION EMBRYOS
NONINVASIVE MEASUREMENT OF PYRUVATE AND GLUCOSE-UPTAKE AND LACTATE PRODUCTION BY SINGLE HUMAN PREIMPLANTATION EMBRYOS
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DOI：
10.1093/oxfordjournals.humrep.a137028
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发表时间：
1990-01-01
影响因子：
6.1
通讯作者：
LEESE, HJ
中科院分区：
文献类型：
--
作者：
GOTT, AL;HARDY, K;LEESE, HJ
采用非侵入性技术测定了40个人类植入前胚胎的丙酮酸和葡萄糖消耗量及乳酸产生量。其中12个胚胎出现异常受精。在28个正常受精的胚胎中，9个（32%）在培养中发育到囊胚期，而其余的在卵裂过程中退化或停滞。在正常胚胎中，在葡萄糖成为胚泡中的主要底物（第6天）之前的早期发育阶段（授精后2-5天）丙酮酸的摄取超过葡萄糖的摄取。在整个发育过程中形成了大量的乳酸盐，从第2.5天的43.6 pmol/胚胎/h上升到第5.5天的95.4 pmol/胚胎/h。这些胚胎的丙酮酸和葡萄糖的摄取和乳酸的产生的值被逮捕低于那些正常发展。在多一个葡萄糖可以产生两摩尔乳酸的基础上，仅50%的所产生的乳酸可以用从培养基中摄取的葡萄糖来解释。这个数字在囊胚中上升到90%。剩余的乳酸必须来自内源性来源，最有可能是糖原。认为人植入前胚胎在体外培养时乳酸的大量产生是对培养条件的一种适应。
The consumption of pyruvate and glucose and the production of lactate by 40 single human preimplantation embryos has been measured using a non-invasive technique. Twelve of the embryos showed abnormal fertilization. Of the 28 normally fertilized embryos, nine (32%) developed to the blastocyst stage in culture while the remainder degenerated or arrested during cleavage. In the normal embryos, pyruvate uptake exceeded that of glucose in the early developmental stages (days 2-5 post-insemination) before glucose became the predominant substrate in the blastocyst (day 6). Considerable quantities of lactate were formed throughout development, rising from a value of 43.6 pmol/embryo/h on day 2.5 to 95.4 pmol/embryo/h on day 5.5. The values of pyruvate and glucose uptake and lactate production of those embryos which arrested were below those which developed normally. On the basis that one more of glucose can give rise to two moles of lactate, only 50% of the lactate produced could be accounted for in terms of glucose uptake from the medium. This figure rose to 90% in the blastocyst. The remaining lactate must be derived from endogenous sources, most probably glycogen. It is proposed that the high production of lactate by human preimplantation embryos in vitro is an adaptation to the conditions of culture.