A dual-color Total Internal Reflection (TIR)-based chip for simultaneous detection of two fluorophores
A dual-color Total Internal Reflection (TIR)-based chip for simultaneous detection of two fluorophores
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DOI：
10.1109/icsens.2008.4716655
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发表时间：
2008-12
期刊：
影响因子：
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通讯作者：
N. Le;D. Dao;R. Yokokawa;J. Wells;S. Sugiyama
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文献类型：
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作者：
N. Le;D. Dao;R. Yokokawa;J. Wells;S. Sugiyama
我们报告了一种新型双色全内反射（TIR）芯片，它可以产生两个重叠的渐逝场，用于同时检测两个荧光团。该芯片采用硅体微机械加工和 PDMS 铸造进行单片制造。我们提出的方法将所有小型化组件（包括两个柱面微透镜、一个棱镜、两个光纤对准槽和两个光纤挡块）集成到一个单片 PDMS 芯片中；因此无需组装，并且避免了错位。我们首先通过同时检测两种荧光染料（即四甲基罗丹明（TMR）和荧光素）来展示该芯片的功能。然后，我们使用该芯片对尼罗红 (NR) 和龙绿 (DG) 荧光珠的混合物进行成像。我们的小型化集成设备可以替代传统的双色全内反射荧光显微镜（双色 TIRFM）系统。它还可以成为用于高灵敏度双色荧光检测的微型全分析系统 (mu-TAS) 的有用组件。
We report a novel, dual-color total internal reflection (TIR)-based chip which can generate two overlapping evanescent fields for simultaneous detection of two fluorophores. The chip was monolithically fabricated using Si bulk micromachining and PDMS casting. Our proposed method integrated all miniaturized components, including two cylindrical microlenses, one prism, two fiber alignment grooves and two fiber stoppers, into one monolithic PDMS chip; thus assembly is unnecessary, and misalignment is avoided. We first demonstrated the capabilities of the chip by detecting simultaneously two fluorescent dyes, namely Tetramethylrhodamine (TMR) and Fluorescein. We then employed the chip to image mixture of Nile-red (NR) and Dragon Green (DG) fluorescent beads. Our miniaturized, integrated device could be an alternative to the conventional dual-color Total Internal Reflection Fluorescent Microscopy (dual-color TIRFM) systems. It could also be a useful component of a micro-Total Analysis System (mu-TAS) for highly-sensitive dual-color fluorescent detection.