Local OPG gene transfer to periodontal tissue inhibits orthodontic tooth movement
Local OPG gene transfer to periodontal tissue inhibits orthodontic tooth movement
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DOI：
10.1177/154405910408301206
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发表时间：
2004-12-01
影响因子：
7.6
通讯作者：
Mitani, H
中科院分区：
文献类型：
--
作者：
Kanzaki, H;Chiba, M;Mitani, H
在此之前，我们发现RANKL在受压牙周韧带细胞中被诱导表达，这促进了正畸牙齿运动中受压侧破骨细胞的发生。我们假设局部的OPG基因转移到牙周组织可以中和机械压缩力诱导的RANKL活性，从而抑制破骨细胞的发生和减少牙齿的运动。6周龄雄性Wistar大鼠上颌第一磨牙通过固定正畸金属丝上颌移动。构建了小鼠OPG表达质粒[pcDNA3.1(+)-mOPG]，并在体外证实了OPG功能蛋白的产生。将含有pcDNA3.1(+)mOPG或PBS的灭活HVJ包膜载体定期注射到上颌第一磨牙腭牙周组织中。当进行这种局部OPG基因转移时，OPG的产生被诱导，破骨细胞的发生被抑制。局部OPG基因转移明显减少牙齿移动。在这项研究中，我们报道了OPG基因转移到牙周组织抑制rankl介导的破骨细胞生成和抑制实验牙齿移动。
Previously, we discovered that RANKL expression is induced in compressed periodontal ligament cells, and that this promotes osteoclastogenesis on the compression side in orthodontic tooth movement. We hypothesized that local OPG gene transfer to the periodontium would neutralize the RANKL activity induced by mechanical compressive force, thereby inhibiting osteoclastogenesis and diminishing tooth movement. The upper first molars of six-week-old male Wistar rats were moved palatally by means of a fixed-orthodontic wire. A mouse OPG expression plasmid [pcDNA3.1(+)-mOPG] was constructed, and the production of functional OPG protein was confirmed in vitro. The inactivated HVJ envelope vector containing pcDNA3.1(+)mOPG or PBS was injected periodically into the palatal periodontal tissue of upper first molars. When this local OPG gene transfer was performed, OPG production was induced, and osteoclastogenesis was inhibited. Local OPG gene transfer significantly diminished tooth movement. In this study, we report that OPG gene transfer to periodontal tissue inhibited RANKL-mediated osteoclastogenesis and inhibited experimental tooth movement.