Repression of the acid ZrfA/ZrfB zinc-uptake system of Aspergillus fumigatus mediated by PacC under neutral, zinc-limiting conditions
Repression of the acid ZrfA/ZrfB zinc-uptake system of Aspergillus fumigatus mediated by PacC under neutral, zinc-limiting conditions
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DOI：
10.2436/20.1501.01.80
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发表时间：
2009-03-01
影响因子：
3.1
通讯作者：
Calera, Jose A.
中科院分区：
文献类型：
--
作者：
Amich, Jorge;Leal, Fernando;Calera, Jose A.
ZrfA 和 ZrfB 转运蛋白是烟曲霉锌吸收系统的组成部分，该系统主要在酸性、锌限制条件下运行。在酸性和中性锌限制培养基中，转录激活剂 ZafA 上调基因 zrfA 和 zrfB 的表达。 zafA 的转录不受 PacC 的影响，PacC 是曲霉中参与调节 pH 稳态的转录调节因子。然而，在中性 pH 条件下，zrfA 和 zrfB 的表达均显着降低。在这项工作中，发现中性和碱性锌限制培养基中 zrfA 和 zrfB 的抑制是由转录调节因子 PacC 介导的。 [国际微生物学，2009； 12（1）：39-47]
The ZrfA and ZrfB transporters are components of a zinc-uptake system of Aspergillus fumigatus that mainly operates under acidic, zinc-limiting conditions. Expression of the genes zrfA and zrfB is up-regulated by the transcriptional activator ZafA in both acidic and neutral, zinc-limiting media. The transcription of zafA is not influenced by PacC, which is the transcriptional regulator involved in regulating pH omeostasis in Aspergillus. However, at neutral pH the expression of both zrfA and zrfB is significantly reduced. In this work, the repression of zrfA and zrfB in neutral and alkaline, zinc-limiting media was found to be mediated by the transcriptional regulator PacC. [Int Microbiol 2009; 12(1):39-47]