Circulation and characterization of seasonal influenza viruses in Cambodia, 2012-2015
Circulation and characterization of seasonal influenza viruses in Cambodia, 2012-2015
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DOI：
10.1111/irv.12647
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发表时间：
2019-06-28
影响因子：
4.4
通讯作者：
Dussart, Philippe
中科院分区：
文献类型：
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作者：
Horwood, Paul F.;Karlsson, Erik A.;Dussart, Philippe
柬埔寨流感样疾病（ILI）哨点监测系统监测流感病毒传播，国家流感中心（NIC）对分离株进行鉴定。季节性流感流行以前的特点是全年活动，雨季（6月至11月）为高峰。我们记录了2012 - 2015年柬埔寨季节性流感的流行情况，并调查了流感分离株的遗传、抗原和抗病毒耐药性特征。患者/方法从柬埔寨11家医院的流感样疾病（ILI）患者中收集呼吸道样本。国家公共卫生研究所和武装部队医学科学研究所进行了一线筛查。检测确认以及遗传、抗原和抗病毒耐药性表征由柬埔寨巴斯德研究所（NIC）进行。世卫组织流感参考和研究合作中心（墨尔本，澳大利亚）进行了额外的病毒表征。结果2012年至2015年期间，向NIC提交了1，238份流感阳性样本。甲型流感（H3 N2）（55.3%）是主要亚型，其次是流感B（30.9%;主要是B/Yamagata谱系）和甲型流感（H1 N1）pdm09（13.9%）。流感病毒的传播开始于2014年和2015年，比以前描述的更早，分别与A（H3N2）分支3C.2a和3C.3a的出现相一致。在此期间，A（H3N2）病毒的抗原性具有高度多样性，B型流感病毒的抗原性具有较小程度的多样性，与北方和南半球疫苗配方存在一些不匹配。所有测试的分离株都对流感抗病毒药物奥司他韦和扎那米韦敏感。结论2012 - 2015年期间，在柬埔寨检测到多种流感类型/亚型的季节性和全年共循环。
Background Influenza virus circulation is monitored through the Cambodian influenza-like illness (ILI) sentinel surveillance system and isolates are characterized by the National Influenza Centre (NIC). Seasonal influenza circulation has previously been characterized by year-round activity and a peak during the rainy season (June-November). Objectives We documented the circulation of seasonal influenza in Cambodia for 2012-2015 and investigated genetic, antigenic, and antiviral resistance characteristics of influenza isolates. Patients/Methods Respiratory samples were collected from patients presenting with influenza-like illness (ILI) at 11 hospitals throughout Cambodia. First-line screening was conducted by the National Institute of Public Health and the Armed Forces Research Institute of Medical Sciences. Confirmation of testing and genetic, antigenic and antiviral resistance characterization was conducted by Institute Pasteur in Cambodia, the NIC. Additional virus characterization was conducted by the WHO Collaborating Centre for Reference and Research on Influenza (Melbourne, Australia). Results Between 2012 and 2015, 1,238 influenza-positive samples were submitted to the NIC. Influenza A(H3N2) (55.3%) was the dominant subtype, followed by influenza B (30.9%; predominantly B/Yamagata-lineage) and A(H1N1)pdm09 (13.9%). Circulation of influenza viruses began earlier in 2014 and 2015 than previously described, coincident with the emergence of A(H3N2) clades 3C.2a and 3C.3a, respectively. There was high diversity in the antigenicity of A(H3N2) viruses, and to a smaller extent influenza B viruses, during this period, with some mismatches with the northern and southern hemisphere vaccine formulations. All isolates tested were susceptible to the influenza antiviral drugs oseltamivir and zanamivir. Conclusions Seasonal and year-round co-circulation of multiple influenza types/subtypes were detected in Cambodia during 2012-2015.