Involvement of EphA2 in the formation of the tail notochord via interaction with ephrinA1
Involvement of EphA2 in the formation of the tail notochord via interaction with ephrinA1
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DOI：
10.1016/s0925-4773(01)00290-8
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发表时间：
2001-04-01
影响因子：
2.6
通讯作者：
Iwakura, Y
中科院分区：
文献类型：
--
作者：
Naruse-Nakajima, C;Asano, M;Iwakura, Y
在胚胎发育过程中，Eph受体参与了细胞间的相互作用。我们用基因捕捉法产生了EphA2突变小鼠。纯合子突变小鼠的尾巴又短又扭曲。用短臂探针进行原位杂交，发现脊索在尾端11.5天至12.5天出现异常分叉。在正常小鼠中，EphA2在尾索顶端表达，而EphA2的一种配体ewitinAl在尾芽中表达。相反，EphA2缺陷的节索细胞广泛分布在尾芽中。这些观察表明，EphA2及其配体通过在细胞表面表达这些分子的细胞之间的排斥信号参与了尾索的定位。(C)2001爱思唯尔爱尔兰科学有限公司。保留所有权利。
Eph receptors have been implicated in cell-to-cell interaction during embryogenesis. We generated EphA2 mutant mice using a gene trap method. Homozygous mutant mice developed short and kinky tails. In situ hybridization using a Brachyury probe found the notochord to be abnormally bifurcated at the caudal end between 11.5 and 12.5 days post coitum. EphA2 was expressed at the tip of the tail notochord, while one of its ligands, ephrinAl, was at the tail bud in normal mice. In contrast, EphA2-deficient notochordal cells were spread broadly into the tail bud. These observations suggest that EphA2 and its ligands are involved in the positioning of the tail notochord through repulsive signals between cells expressing these molecules on the surface. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.