CELL-FREE TRANSLATION OF MURINE CORONAVIRUS RNA
CELL-FREE TRANSLATION OF MURINE CORONAVIRUS RNA
复制标题
DOI：
10.1128/jvi.43.3.905-913.1982
复制
发表时间：
1982-01-01
影响因子：
5.4
通讯作者：
BOND, CW
中科院分区：
文献类型：
--
作者：
LEIBOWITZ, JL;WEISS, SR;BOND, CW
通过无细胞翻译研究了细胞内鼠肝炎病毒特异性亚基因组 RNA 种类和鼠肝炎病毒颗粒 RNA 的编码分配。通过琼脂糖凝胶电泳部分纯化6种鼠肝炎病毒特异性亚基因组RNA，并在mRNA依赖性兔网织红细胞裂解液中进行翻译，并通过凝胶电泳、免疫沉淀和胰蛋白酶肽图谱对无细胞翻译产物进行表征。 RNA 7 编码核衣壳蛋白，RNA 6 编码 E1 蛋白，RNA 3 编码 E2 蛋白，RNA 2 编码 35,000 道尔顿非结构蛋白。基因组 RNA 指导 3 种结构相关的多肽（MW 大于 200,000）的无细胞合成。
The coding assignments of the intracellular murine hepatitis virus-specific subgenomic RNA species and murine hepatitis virion RNA were investigated by cell-free translation. The 6 murine hepatitis virus-specific subgenomic RNA were partially prufied by agarose gel electrophoresis and translated in an mRNA-dependent rabbit reticulocyte lysate, and the cell-free translation products were characterized by gel electrophoresis, immunoprecipitation and tryptic peptide mapping. RNA 7 codes for the nucleocapsid protein, RNA 6 codes for the E1 protein, RNA 3 codes for the E2 protein and RNA 2 codes for a 35,000-dalton nonstructural protein. Genomic RNA directs the cell-free synthesis of 3 structurally related polypeptides of > 200,000 in MW.