Brain-derived neurotrophic factor in astrocytes, oligodendrocytes, and microglia/macrophages after spinal cord injury
Brain-derived neurotrophic factor in astrocytes, oligodendrocytes, and microglia/macrophages after spinal cord injury
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DOI：
10.1006/nbdi.2000.0318
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发表时间：
2000-12-01
影响因子：
6.1
通讯作者：
Black, IB
中科院分区：
文献类型：
--
作者：
Dougherty, KD;Dreyfus, CF;Black, IB
最近的研究表明，损伤的成年脊髓对脑源性神经营养因子（BDNF）和神经营养因子-3（NT-3）的反应是增强神经元存活和轴突再生。脊髓中潜在的神经营养因子来源和细胞定位在很大程度上是不确定的。我们检测了胶质细胞BDNF在正常脊髓中的定位和在活体损伤后的时空分布。我们使用双重免疫标记法检测星形胶质细胞中的BDNF和胶质细胞酸性蛋白（GFAP），少突胶质细胞中的腺瘤性息肉病结肠肿瘤抑制蛋白（APC）或小胶质细胞/巨噬细胞中的III型CDH受体（OX 42）。在正常脊髓中，小的星形胶质细胞和小胶质细胞/巨噬细胞和大多数少突胶质细胞的亚群表现出BDNF免疫反应。损伤后，随着时间的推移，BDNF免疫阳性星形胶质细胞和小胶质细胞/巨噬细胞的数量在损伤部位急剧增加。大多数少突胶质细胞含有BDNF损伤后1天和1周，但APC阳性细胞在损伤后6周的损伤部位基本上没有。胶质细胞BDNF免疫标记也检查了10和20毫米的伤口。在距离损伤10毫米处，星形胶质细胞和小胶质细胞/巨噬细胞的BDNF免疫标记与损伤处的BDNF免疫标记在所有检查时间都相似。损伤后20毫米，BDNF在所有三种胶质细胞亚型中的定位与对照组相似，与损伤后时间无关。我们的研究结果表明，在正常成人脊髓，星形胶质细胞，少突胶质细胞和小胶质细胞/巨噬细胞发挥作用，在当地的营养素的可用性和营养素介导的损伤和愈合反应直接内和周围的伤口部位。(C)北京大学出版社.
Recent studies suggest that the injured adult spinal cord responds to brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT3) with enhanced neuron survival and axon regeneration. Potential neurotrophin sources and cellular localization in spinal cord are largely undefined. We examined glial BDNF localization in normal cord and its temporospatial distribution after injury in viva We used dual immunolabeling for BDNF and glial fibrillary acidic protein (GFAP) in astrocytes, adenomatous polyposis coli tumor suppressor protein (APC) for oligodendrocytes or type III CDH receptor (OX42) for microglia/macrophages. In normal cord, small subsets of astrocytes and microglia/macrophages and most oligodendrocytes exhibited BDNF-immunoreactivity. Following injury, the number of BDNF-immunopositive astrocytes and microglia/macrophages increased dramatically at the injury site over time. Most oligodendrocytes contained BDNF 1 day and 1 week following injury, but APC-positive cells were largely absent at the injury site 6 weeks postinjury. Glial BDNF-immunolabeling was also examined 10 and 20 mm from the wound. Ten millimeters from the lesion, astrocyte and microglia/macrophage BDNF-immunolabeling resembled that at the injury at all times examined. Twenty millimeters from injury, BDNF localization in all three glial subtypes resembled controls, regardless of time postlesion. Our findings suggest that in normal adult cord, astrocytes, oligodendrocytes, and microglia/macrophages play roles in local trophin availability and in trophin-mediated injury and healing responses directly within and surrounding the wound site. (C) 2000 Academic Press.