Changes in esterified pectins during development in the flax stems and leaves
Changes in esterified pectins during development in the flax stems and leaves
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DOI：
10.1016/j.carbpol.2005.12.041
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发表时间：
2006-07-25
影响因子：
11.2
通讯作者：
Courtois, J.
中科院分区：
文献类型：
--
作者：
Bedouet, L.;Denys, E.;Courtois, J.
以冬用亚麻（Linun usitatissimum L.）幼苗为材料，在休眠期、伸长期、成熟期和衰老期沿着采收，用酸法提取茎、叶中的果胶并进行分析。单糖分析表明，存在的特定糖的果胶提取物。阿拉伯半乳聚糖多糖与果胶的共提取是可能的，而半纤维素材料的提取似乎不太可能在木糖和法卡糖的情况下。从茎中提取的果胶的产量随时间变化，并且沿着发育降低，在休眠阶段为7%，在衰老阶段为2%，而叶导致果胶的恒定产量（类似于4%）。在茎中，果胶半乳糖醛酸的成熟过程中，从53%在休眠到66%在衰老过程中。在叶中，果胶比茎中的酸性更强，并且组成和大小的沿着发育变化不显著。在发育过程中，酸溶性果胶的甲基化程度很高（80-100%），变化适度，而乙酰化程度很重要（23-40%），略有增加。在全球范围内，从叶果胶的修改是更有限的茎相比，和温和的酸提取表明，高分子量酯化果胶存在或仍然合成亚麻，直到衰老。(c)2006爱思唯尔有限公司保留所有权利。
Winter oil flax species (Linun usitatissimum L) seedlings were harvested along the development at the dormancy, elongation, maturation and senescence periods, and pectins in stems and leaves were extracted in acid and analysed. The monosaccharides analyses indicated presence of the specific sugar of pectins in the extracts. Co-extraction of arabinogalactan polysaccharides with pectins was possible whereas extraction of hemicellulose material seemed unlikely in regard to the absence of xylose and facose. The yields of pectins extraction from the stems changed over time and decreased along the development, 7% at the dormancy stage and 2% at senescence, whereas the leaves led to constant yields of pectins over ageing (similar to 4%). In the stems, pectins incorporated galacturonic acid as maturation process, from 53% at dormancy to 66% during senescence. In the leaves, the pectins appeared more acidic than in the stems, and changes along development in composition and size were not significant. During development, methylation of acid-soluble pectins was high (80-100%) and varied moderately, whereas the degree of acetylation was important (23-40%) and increased slightly. Globally, modifications of pectins from leaves were more limited compare to stems, and mild acid extraction indicated that high molecular weight esterified pectins are present or still synthesised in flax until senescence. (c) 2006 Elsevier Ltd. All rights reserved.