Hydrogen sulfide inhalation decreases early blood-brain barrier permeability and brain edema induced by cardiac arrest and resuscitation
Hydrogen sulfide inhalation decreases early blood-brain barrier permeability and brain edema induced by cardiac arrest and resuscitation
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吸入硫化氢可降低心脏骤停和复苏引起的早期血脑屏障通透性和脑水肿
DOI：
10.1038/jcbfm.2014.223
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发表时间：
2015-03-01
影响因子：
6.3
通讯作者：
Zhang, Bing
中科院分区：
文献类型：
--
作者：
Geng, Yingjie;Li, Eerdunmutu;Zhang, Bing
硫化氢（H2S）对心脏骤停（CA）和心肺复苏（CPR）后血脑屏障（BBB）和脑水肿的影响尚不清楚。我们研究了外源性80-p. p.m. H2S气体对血脑屏障、脑含水量、神经学结果和CA和CPR后存活率的影响。大鼠心室颤动6分钟诱发CA后心脏复苏。结果显示，在复苏后24小时，吸入80-p. p.m. H2S显著降低了皮层和海马中BBB的通透性。硫化氢还减轻了大脑皮层和海马的脑水肿，改善了神经功能缺损评分和胶带拆除试验评估的神经功能结局，并提高了14天的生存率。硫化氢还减弱CA和CPR诱导的基质金属蛋白酶-9（MMP-9）活性和血管内皮生长因子（VEGF）表达的增加，并增加血管生成素-1（Ang-1）的表达。这些结果表明，心肺复苏后立即吸入80-p. p.m. H2S可减弱BBB通透性和脑水肿，并改善CA后大鼠的神经功能结局和14天存活率。H2S的治疗益处可能与MMP-9和VEGF表达的抑制以及Ang-1表达的增加有关。
The effects of hydrogen sulfide (H2S) on blood brain barrier (BBB) and brain edema after cardiac arrest (CA) and cardiopulmonary resuscitation (CPR) remain poorly understood. We investigated the effects of exogenous 80-p.p.m. H2S gas on BBB, brain water content, neurologic outcome, and survival rate after CA and CPR. Cardiopulmonary resuscitation followed CA induced in rats by ventricular fibrillation for 6 minutes. Results show that inhalation of 80-p.p.m. H2S significantly reduced the permeability of the BBB in both in the cortex and hippocampus at 24 hours after resuscitation. Hydrogen sulfide also lessened brain edema in the cortex and hippocampus, ameliorated neurologic outcome as evaluated by neurologic deficit score and tape removal test, and improved the 14-day survival rate. Hydrogen sulfide also attenuated CA and CPR-induced increases of matrix metalloproteinase-9 (MMP-9) activity and vascular endothelial growth factor (VEGF) expression, and increased the expression of angiogenin-1 (Ang-1). These results indicate that inhalation of 80-p.p.m. H2S immediately after CPR attenuated BBB permeability and brain edema, and improved neurologic outcome and 14-day survival of rats after CA. The therapeutic benefits of H2S could be associated with suppression of MMP-9 and VEGF expression and increased expression of Ang-1.