Identification of a SCAR marker linked to a recessive male sterile gene (Tems) and its application in breeding of marigold (Tagetes erecta)
Identification of a SCAR marker linked to a recessive male sterile gene (Tems) and its application in breeding of marigold (Tagetes erecta)
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DOI：
10.1111/j.1439-0523.2008.01536.x
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发表时间：
2009-02-01
期刊：
影响因子：
2
通讯作者：
Bao, M. Z.
中科院分区：
文献类型：
--
作者：
He, Y. H.;Ning, G. G.;Bao, M. Z.
用万寿菊雄性不育系M525 A与自交系f53 f杂交，构建了167株的F-2分离群体。M525 A的雄性不育性受隐性基因Tems控制。利用简单重复序列（ISSR）和序列相关扩增多态性（SRAP）技术，结合群体分离分析，筛选出与该性状连锁的分子标记。通过对38个ISSR引物和170个SRAP引物组合的分析，仅筛选出1个与目标性状紧密连锁的SRAP标记，并成功转化为距离Tems位点2.4cM以内的序列特征扩增区（SCAR）标记。该标记与其他五个两型线进行验证，在每种情况下，雄性可育植株被可靠地鉴定。因此，该SCAR标记允许有效的标记辅助选择雄性不育个体的万寿菊育种计划，将大大促进F-1品种的选育。
In marigold, an F-2 segregation population of 167 plants was constructed from a cross of a line (M525A) carrying the male sterility trait x an inbred line (f53f). In line M525A, the male sterility trait was controlled by the recessive gene, Tems. The intersimple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) techniques combined with bulked segregant analysis were used to develop markers linked to the trait. From a survey of the 38 ISSR primers and 170 SRAP primer combinations, only one SRAP marker that was closely linked to the target trait was identified and successfully converted into sequence characterized amplified region (SCAR) marker that was located within 2.4 cM from Tems locus. The marker was validated with five other two-type lines and in each case the male fertile plants were reliably identified. This SCAR marker therefore permits the efficient marker-assisted selection of male sterile individuals in breeding programmes of marigold and will greatly facilitate the breeding of F-1 cultivars.