HYPOXIA-INDUCED HYPOTHERMIA MEDIATED BY NORADRENALINE AND NITRIC OXIDE IN THE ROSTROMEDIAL PREOPTIC AREA
HYPOXIA-INDUCED HYPOTHERMIA MEDIATED BY NORADRENALINE AND NITRIC OXIDE IN THE ROSTROMEDIAL PREOPTIC AREA
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DOI：
10.1016/j.neuroscience.2011.01.056
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发表时间：
2011-04-14
期刊：
影响因子：
3.3
通讯作者：
Osaka, T.
中科院分区：
文献类型：
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作者：
Osaka, T.
中枢一氧化氮（NO）在缺氧引起的体温降低和下丘脑视前区（POA）微量注射去甲肾上腺素（NA）引起的体温降低中起重要作用。在这里，我测试的假设，激活肾上腺素能受体和NO在rostromedial POA参与缺氧诱导的低温在氯醛糖麻醉，神经肌肉阻断，人工通气大鼠。低氧通气（10%O-2- 90%N-2，5分钟）引起的尾部皮肤温度的增加和结肠温度的降低，虽然这些变化发生在30秒至7分钟后，大鼠返回到室内空气通风。这些反应消除了先前的双边横断颈动脉窦神经，但不是由双边颈部迷走神经切断术，这表明参与激活的颈动脉化学感受器在缺氧通气诱导的低温。这种反应也大大减弱了微量注射NO合成酶（NOS）抑制剂，N-G-单甲基-L-精氨酸（L-NMMA，25 nmol），但不是由其无活性的对映体，N-G-单甲基-D-精氨酸（D-NMMA，25 nmol），到NA敏感的，hypothera-inducing网站在rostromedial POA。用α（1）-肾上腺素受体阻滞剂哌唑嗪（50 pmol）预处理，而不是溶剂盐水，也大大减弱了低氧通气诱导的热损失反应。这些结果表明，这种缺氧诱导的体温降低介导，至少部分，通过激活α（1）-肾上腺素受体和NOS在吻内侧POA。(C)2011年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Central nitric oxide (NO) has an important role in hypothermia induced by hypoxia as well as in that elicited by noradrenaline (NA) microinjected into the rostromedial preoptic area (POA) of the hypothalamus. Here, I tested the hypothesis that activation of adrenoceptors and NO in the rostromedial POA is involved in hypoxia-induced hypothermia in urethane-chloralose-anesthetized, neuromuscularly blocked, artificially ventilated rats. Hypoxic ventilation (10% O-2-90% N-2, 5 min) evoked an increase in the tail skin temperature and a decrease in the colonic temperature, though these changes occurred at 30 s to 7 min after returning the rats to ventilation with room air. These responses were eliminated by prior bilateral transection of the carotid sinus nerves, but not by bilateral cervical vagotomy, suggesting the involvement of activated carotid chemoreceptors in the hypoxic ventilation-induced hypothermia. Such responses were also greatly attenuated by the microinjection of an NO synthase (NOS) inhibitor, N-G-monomethyl-L-arginine (L-NMMA, 25 nmol), but not by that of its inactive enantiomer, N-G-monomethyl-D-arginine (D-NMMA, 25 nmol), into the NA-sensitive, hypothermia-inducing site in the rostromedial POA. Pretreatment with the alpha(1)-adrenoceptor blocker prazosin (50 pmol), but not vehicle saline, also greatly attenuated the hypoxic ventilation-induced heat loss responses. These results suggest that this hypoxia-induced hypothermia was mediated, at least in part, by activation of alpha(1)-adrenoceptors and NOS in the rostromedial POA. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.