Reduction of infection-stimulated periapical bone resorption by the biological response modifier PGG glucan.
Reduction of infection-stimulated periapical bone resorption by the biological response modifier PGG glucan.
复制标题
通过生物反应调节剂 PGG 葡聚糖减少感染刺激的根尖周骨吸收。
DOI：
10.1177/00220345950740010701
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发表时间：
1995
影响因子：
7.6
通讯作者：
Niederman,R
中科院分区：
文献类型：
--
作者：
Stashenko,P;Wang,CY;Riley,E;Wu,Y;Ostroff,G;Niederman,R
牙髓和牙周病是细菌感染，导致局部结缔组织和骨破坏。宿主对这些感染的有效抵抗主要由嗜中性粒细胞和其他吞噬细胞介导。PGG葡聚糖（聚-β1-6-葡三糖基-β1-3-吡喃葡萄糖葡聚糖）是一种生物反应调节剂，可刺激中性粒细胞的产生并上调其吞噬和杀菌活性。在本研究中，在体内模型中测试PGG葡聚糖对感染刺激的牙槽骨吸收的影响。在Sprague-Dawley大鼠中通过手术牙髓暴露和随后的口腔环境感染诱导根尖周骨吸收。在牙髓暴露程序前一天和之后第2、4、6、9、11、13、16和18天，对动物皮下施用PGG葡聚糖（0.5 mg/kg）或盐水（对照）。PGG葡聚糖增加了循环中性粒细胞和单核细胞的数量，并使中性粒细胞吞噬活性增加了约两倍。通过放射学（-48.0%; p < 0.001）和组织形态测量学（第一和第二磨牙分别为-40.8%和-42.4%; p < 0.01）确定，PGG葡聚糖治疗动物的感染刺激根尖周骨吸收显著低于对照动物。PGG葡聚糖治疗的动物也有较少的软组织破坏，如减少牙髓坏死所示。PGG葡聚糖处理动物的第一磨牙牙髓中只有3.3%表现出完全坏死，而对照动物的牙髓中有40.6%表现出完全坏死。最后，PGG葡聚糖在体外对PTH或IL-1刺激的骨吸收没有影响。这些发现支持了这样的概念，即增强中性粒细胞内源性抗菌机制的生物反应调节剂可以减少体内感染刺激的牙槽骨和软组织破坏。
Pulpal and periodontal diseases are bacterial infections which result in local connective tissue and bone destruction. Effective host resistance to these infections is primarily mediated by neutrophils and other phagocytic cells. PGG glucan (poly-β1-6-glucotriosyl-β1-3-glucopyranose glucan) is a biological response modifier which stimulates the production of neutrophils and upregulates their phagocytic and bactericidal activity. In the present studies, the effect of PGG glucan on infection-stimulated alveolar bone resorption was tested in an in vivo model. Periapical bone resorption was induced in Sprague-Dawley rats by surgical pulp exposure and subsequent infection from the oral environment. Animals were administered PGG glucan (0.5 mg/kg) or saline (control) subcutaneously the day before and on days 2, 4, 6, 9, 11, 13, 16, and 18 following the pulp exposure procedure. PGG glucan enhanced the number of circulating neutrophils and monocytes and increased neutrophil phagocytic activity approximately two-fold. PGG glucan-treated animals had significantly less infection-stimulated periapical bone resorption than control animals, as determined radiographically (-48.0%; p < 0.001) and by histomorphometry (-40.8% and -42.4% for first and second molars, respectively; p < 0.01). PGG glucan-treated animals also had less soft tissue destruction, as indicated by decreased pulpal necrosis. Only 3.3% of first molar pulps from PGG glucan-treated animals exhibited complete necrosis, as compared with 40.6% of pulps from controls. Finally, PGG glucan had no effect on either PTH- or IL-1-stimulated bone resorption in vitro. These findings support the concept that a biological response modifier which enhances endogenous antibacterial mechanisms in neutrophils can decrease infection-stimulated alveolar bone and soft tissue destruction in vivo.