Label-free protein biosensor based on aptamer-modified carbon nanotube field-effect transistors
Label-free protein biosensor based on aptamer-modified carbon nanotube field-effect transistors
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DOI：
10.1021/ac060830g
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发表时间：
2007-01-15
影响因子：
7.4
通讯作者：
Tamiya, Eiichi
中科院分区：
文献类型：
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作者：
Maehashi, Kenzo;Katsura, Taiji;Tamiya, Eiichi
我们基于适体修饰的碳纳米管场效应晶体管（CNT-FET）制造了无标记蛋白质生物传感器，用于检测免疫球蛋白E（IgE）。在CNT通道上共价固定5 '-氨基修饰的45-mer适体之后，真实的实时监测CNT-FET的电性质。在各种浓度的目标IgE的引入引起的源极-漏极电流急剧下降，并在较低的浓度下观察到逐渐饱和。在适体修饰的CNT-FET上引入IgE之前和之后的净源极-漏极电流的量作为IgE浓度的函数增加。IgE的检测限确定为250 pM。我们还准备了CNT-FET生物传感器，使用单克隆抗体对IgE（IgE-mAb）。比较了适体和抗体修饰的CNT-FET的电性能。适体修饰的CNT-FET的性能提供了比在类似条件下使用IgE-mAb修饰的CNT-FET获得的结果更好的结果。因此，我们认为，适配子修饰的碳纳米管场效应晶体管是有前途的候选人，为开发无标记的蛋白质生物传感器。
We have fabricated label-free protein biosensors based on aptamer-modified carbon nanotube field-effect transistors (CNT-FETs) for the detection of immunoglobulin E (IgE). After the covalent immobilization of 5'-amino-modified 45-mer aptamers on the CNT channels, the electrical properties of the CNT-FETs were monitored in real time. The introduction of target IgE at various concentrations caused a sharp decrease in the source-drain current, and a gradual saturation was observed at lower concentrations. The amount of the net source-drain current before and after IgE introduction on the aptamer-modified CNT-FETs increased as a function of IgE concentration. The detection limit for IgE was determined as 250 pM. We have also prepared CNT-FET biosensors using a monoclonal antibody against IgE (IgE-mAb). The electrical properties of the aptamer- and antibody-modified CNT-FETs were compared. The performance of aptamer-modified CNT-FETs provided better results than the ones obtained using IgE-mAb-modified CNT-FETs under similar conditions. Thus, we suggest that the aptamer-modified CNT-FETs are promising candidates for the development of label-free protein biosensors.