Evolution of Nanoparticle Protein Corona across the Blood-Brain Barrier
Evolution of Nanoparticle Protein Corona across the Blood-Brain Barrier
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DOI：
10.1021/acsnano.8b03500
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发表时间：
2018-07-01
期刊：
影响因子：
17.1
通讯作者：
Re, Francesca
中科院分区：
文献类型：
--
作者：
Cox, Alysia;Andreozzi, Patrizia;Re, Francesca
工程纳米粒子提供了改善药物通过生物屏障的运输和递送的机会，利用离开血液循环并穿过内皮血管床（包括血脑屏障（BBB））以到达目标的可能性。众所周知，纳米粒子在与生物体液接触时在其表面聚集分子，形成“蛋白质电晕”，这可以影响它们的命运和治疗/诊断性能，但迄今为止尚未收集到有关电晕跨越屏障演化的信息。使用血脑屏障和金纳米粒子的细胞模型，我们表明，在从“血液”到“大脑”一侧的过程中，电晕的成分经历了剧烈的定量和定性分子修饰，而一旦超出血脑屏障，它就保持稳定。因此，我们证明纳米颗粒电晕在穿过血脑屏障时会动态地、剧烈地演化，并且一旦超出靶器官的屏障，其初始组成并不能预测纳米颗粒的命运和性能。
Engineered nanoparticles offer the chance to improve drug transport and delivery through biological barriers, exploiting the possibility to leave the blood circulation and traverse the endothelial vascular bed, blood-brain barrier (BBB) included, to reach their target. It is known that nanoparticles gather molecules on their surface upon contact with biological fluids, forming the "protein corona", which can affect their fate and therapeutic/diagnostic performance, yet no information on the corona's evolution across the barrier has been gathered so far. Using a cellular model of the BBB and gold nanoparticles, we show that the composition of the corona undergoes dramatic quantitative and qualitative molecular modifications during passage from the "blood" to the "brain" side, while it is stable once beyond the BBB. Thus, we demonstrate that the nanoparticle corona dynamically and drastically evolves upon crossing the BBB and that its initial composition is not predictive of nanoparticle fate and performance once beyond the barrier at the target organ.