PATTERNS OF OVARIAN CELL-PROLIFERATION IN RATS DURING THE EMBRYONIC PERIOD AND THE FIRST 3 WEEKS POSTPARTUM
PATTERNS OF OVARIAN CELL-PROLIFERATION IN RATS DURING THE EMBRYONIC PERIOD AND THE FIRST 3 WEEKS POSTPARTUM
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DOI：
10.1095/biolreprod53.5.1208
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发表时间：
1995-11-01
影响因子：
3.6
通讯作者：
DESANTI, AM
中科院分区：
文献类型：
--
作者：
HIRSHFIELD, AN;DESANTI, AM
本研究的目的是表征卵泡发生最早阶段（发生在大鼠胚胎期和产后第一周）的卵巢细胞增殖模式。连续输注大鼠 [H-3] 胸苷 (H-3-TdR) 或溴脱氧尿苷 (BrdU)，并通过放射自显影或免疫组织化学观察正在合成 DNA 的细胞。在研究期间，细胞增殖模式发生了巨大变化。间充质细胞在胚胎和新生儿卵巢中广泛增殖，但随着卵泡的形成，它们的生长分数急剧下降。卵巢髓质的上皮细胞在胚胎期第12天左右就离开了细胞周期，出生后一融入卵泡就恢复增殖，朝向器官皮质的上皮细胞继续增殖，直到胚胎期后期；它们显然在出生时就处于静止状态，并入卵泡并没有使它们脱离静止状态。卵泡形成后，细胞增殖的模式继续发生变化。产后 5 天，大约 36% 的最小卵泡（横截面中有 1-8 个颗粒细胞）至少有 1 个颗粒细胞，在 3H-TdR 输注 24 小时后被标记；到第 20 天，只有 14% 的卵泡被标记，到第 30 天，只有 4.4% 被标记。
The purpose of this study was to characterize the patterns of ovarian cell proliferation during the earliest stages of folliculogenesis, which occur in the embryonic period and the first weeks postpartum in rats. Rats were given continuous infusions of [H-3]thymidine (H-3-TdR) or bromodeoxyuridine (BrdU), and cells that were synthesizing DNA were visualized by autoradiography or immunohistochemistry. There were dramatic changes in the patterns of cell proliferation during the period studied. Mesenchymal cells proliferated extensively in the embryonic and neonatal ovary, but their growth fraction declined precipitously as follicles formed. Epithelial cells in the medulla of the ovary left the cell cycle at about embryonic Day 12, then resumed proliferation as soon as they were incorporated into follicles just after birth, Epithelial cells towards the cortex of the organ continued to proliferate until late in the embryonic period; they apparently became quiescent around the time of birth, and incorporation into follicles did not release them from their quiescent state, After the follicles had formed, patterns of cell proliferation continued to change. At 5 days postpartum, approximately 36% of the smallest follicles (1-8 granulosa cells in cross section) had at least 1 granulosa cell that was labeled following a 24-h infusion of 3H-TdR; by Day 20 only 14% of these follicles were labeled, and by Day 30 only 4.4% were labeled.