Temporal and spatially controllable cell encapsulation using a water-soluble phospholipid polymer with phenylboronic acid moiety
Temporal and spatially controllable cell encapsulation using a water-soluble phospholipid polymer with phenylboronic acid moiety
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DOI：
10.1016/j.biomaterials.2006.12.017
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发表时间：
2007-04-01
期刊：
影响因子：
14
通讯作者：
Ishihara, Kazuhiko
中科院分区：
文献类型：
--
作者：
Konno, Tomohiro;Ishihara, Kazuhiko
本研究报道了基于水溶性磷脂聚合物的时间和空间可控的细胞包封。磷脂聚合物，即，合成了聚（2-甲基丙烯酰氧乙基磷酰胆碱-co-甲基丙烯酸正丁酯-co-对乙烯基苯硼酸）（PMBV）。将水溶性PMBV与其他含多价醇基的水溶性聚合物如聚乙烯醇（PVA）制备了一系列水凝胶。PMBV/PVA水凝胶不仅在水中形成，而且在细胞培养基中形成，并且通过过量添加低分子量二价羟基化合物（例如D-葡萄糖）解离。将PMBV/PVA水凝胶作为细胞容器，其具有用于活细胞的可逆包封的三维基质而没有任何反应。由于在细胞培养基中PMBV和PVA的均匀凝胶形成，使用水凝胶可以实现均匀的细胞接种。将成纤维细胞包封在PMBV/PVA水凝胶中并维持1周。在PMBV/PVA水凝胶解离后，将细胞接种在常规组织培养聚苯乙烯上。细胞在平板上正常粘附和增殖。也就是说，PMBV/PVA水凝胶将可用作细胞容器，其可以维持细胞而对截留的细胞没有任何显著的不利影响。(c)2006爱思唯尔有限公司保留所有权利。
Temporal and spatially controllable cell encapsulation based on a water-soluble phospholipid polymer is reported in this study. Phospholipid polymers, i.e., poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-vinylphenylboronic acid) (PMBV), were synthesized. A series of hydrogels was prepared between the water-soluble PMBV and other water-soluble polymers having multi-valent alcoholic groups, such as poly(vinyl alcohol) (PVA). The PMBV/PVA hydrogels were formed not only in water, but also in a cell culture medium, and dissociated by the excess addition of low molecular weight di-valent hydroxyl compounds, such as D-glucose. The PMBV/PVA hydrogel was applied as a cell-container which has three-dimensional matrices for the reversible encapsulation of living cells without any response in it. Uniform cell seeding can be achieved using the hydrogels due to the homogenous gel formation of PMBV and PVA in the cell culture medium. Fibroblast cells were encapsulated in the PMBV/PVA hydrogel and maintained for I week. After dissociation of the PMBV/PVA hydrogel, the cells were seeded on conventional tissue culture polystyrene. The cells adhered and proliferated as usual on the plate. That is, the PMBV/PVA hydrogel will be useful as a cell-container, which can maintain the cells without any significant adverse effect on the entrapped cells. (c) 2006 Elsevier Ltd. All rights reserved.