Studies of the cellular uptake of hydrogel nanospheres and microspheres by phagocytes, vascular endothelial cells, and smooth muscle cells.
Studies of the cellular uptake of hydrogel nanospheres and microspheres by phagocytes, vascular endothelial cells, and smooth muscle cells.
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DOI：
10.1002/jbm.a.31734
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发表时间：
2009-03-15
影响因子：
4.9
通讯作者：
Tang, Liping
中科院分区：
文献类型：
--
作者：
Nguyen, Kytai Truong;Shukla, Kajal P.;Moctezuma, Miriam;Braden, Arthur R. C.;Zhou, Jun;Hu, Zhibing;Tang, Liping
Intensive research efforts have been placed on the development of nanospheres for targeted drug delivery for treating a variety of diseases, including coronary restenosis, cancer, and inflammatory reactions. Although most of these drug-bearing spheres are delivered via intravenous administration, little is known about the effect of sphere physical characteristics on the responses of vascular and blood cells. To find the answer, this work was aimed to investigate the cellular uptake of nano- (100nm) and micro-sized hydrogel spheres (1 µm) made of poly (N-isopropylacrylamide) by vascular cells and phagocytes under various flow conditions in vitro. We found that the cellular uptake of nanospheres depended on incubation times and sphere concentrations as well as on the introduced shear stress levels of the medium. Measurements of the intracellular released fluorescence and confocal fluorescence microscopy revealed that nanospheres were internalized by endothelial cells and smooth muscle cells more than microspheres, whereas microspheres were rapidly taken up by phagocytes, especially at high concentration. Our results strongly suggest that hydrogel nanospheres are more effective as an intra-vascular delivery system compared to microspheres in the term of vascular cellular uptake and biocompatibility.
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影响因子：
14
作者：
Eniola, AO;Hammer, DA
通讯作者：
Hammer, DA
影响因子：
3.8
作者：
Blackwell, JE;Dagia, NM;Goetz, DJ
通讯作者：
Goetz, DJ
影响因子：
10.8
作者：
Fishbein, I;Chorny, M;Golomb, G
通讯作者：
Golomb, G
影响因子：
3.7
作者：
Desai, MP;Labhasetwar, V;Amidon, GL
通讯作者：
Amidon, GL
影响因子：
15.6
作者：
Pelton, R
通讯作者：
Pelton, R