Distribution and immunoelectron microscopic localization of laminin, a noncollagenous basement membrane glycoprotein.
Distribution and immunoelectron microscopic localization of laminin, a noncollagenous basement membrane glycoprotein.
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层粘连蛋白（一种非胶原基底膜糖蛋白）的分布和免疫电子显微镜定位。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
S. Katz
中科院分区：
文献类型：
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作者：
J. Foidart;E. Bere;M. Yaar;S. Rennard;M. Gullino;G. Martin;S. Katz
层粘连蛋白是从可移植性小鼠肿瘤基底膜中分离出来的一种非胶原糖蛋白。纯化的层粘连蛋白抗体不与其他已知的BM抗原交叉反应，包括IV型胶原蛋白、纤连蛋白、大疱性类天疱疮抗原和BM蛋白聚糖。使用免疫荧光，层粘连蛋白定位于那些人，鸡，豚鼠，牛，猴，大鼠和小鼠组织的BM区。培养的上皮细胞和内皮细胞合成层粘连蛋白，而间充质细胞不合成。通过免疫电镜，层粘连蛋白定位于人表皮BM和小鼠食管上皮BM的透明层。层粘连蛋白在不同组织和物种中以及在胚胎发育的早期阶段的广泛分布表明层粘连蛋白是基底膜的普遍存在的组分。
Laminin is a noncollagenour glycoprotein isolated from a transplantable mouse tumor producting basement membrane (BM). Purified antibodies to laminin do not cross-react with other known BM antigens including type IV collagen, fibronectin, bullous pemphigoid antigen, and a BM proteoglycan. Using immunofluorescence, laminin is localized in the BM zones of those human, chick, guinea pig, bovine, monkey, rat, and mouse tissues examined. Epithelial and endothelial cells in culture synthesize laminin while mesenchymal cells do not. By immunoelectron microscopy, laminin was localized to the lamina lucida of human epidermal BM and of mouse esophagus epithelial BM. The wide distribution of laminin among diverse tissues and species, and in early stages of embryonic development suggests that laminin is an ubiquitous component of basement membranes.