CHANGES IN TYPE OF COLLAGEN SYNTHESIZED AS CLONES OF CHICK CHONDROCYTES GROW AND EVENTUALLY LOSE DIVISION CAPACITY
CHANGES IN TYPE OF COLLAGEN SYNTHESIZED AS CLONES OF CHICK CHONDROCYTES GROW AND EVENTUALLY LOSE DIVISION CAPACITY
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DOI：
10.1073/pnas.73.5.1674
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发表时间：
1976-01-01
影响因子：
11.1
通讯作者：
MILLER, EJ
中科院分区：
文献类型：
--
作者：
MAYNE, R;VAIL, MS;MILLER, EJ
分离鸡胚胎软骨细胞的克隆，随着克隆的生长，胶原的生物合成随之而来，最终失去分裂能力。对每一次继代培养直到细胞衰老时的胶原类型的分析表明，从软骨特有的II型胶原(链组成[α1(II)]3)到I型胶原(链组成[α1(I)]2α2)和I型三聚体(链组成[α1(I)]3)的混合物的合成发生了变化。鸡软骨细胞表型的表达在体外是不稳定的，以前大量培养软骨细胞的实验不能用成纤维细胞的过度生长来解释。由于软骨细胞在5-溴-2‘’-脱氧尿苷中生长几天后，观察到相似的形态变化和胶原生物合成的相似转换，这种类似物的生长加速了这些变化，最终导致细胞衰老。
Clones of embryonic chick chondrocytes were isolated and collagen biosynthesis followed as the clones grow and eventually lose division capacity. Analysis of collagen type at each successive subculture until the time of cellular senescence showed that a change in synthesis occurs from the cartilage-specific Type II collagen (chain composition [.alpha.1(II)]3) to mixture of Type I collagen (chain composition [.alpha.1(I)]2.alpha.2) and the Type I trimer (chain composition [.alpha.1(I)]3). The expression of the chick chondrocyte phenotype is unstable in vitro, and previous experiments with mass cultures of chondrocytes cannot be accounted for by overgrowth of fibroblasts. Since similar morphological changes and a similar switching in collagen biosynthesis were observed after growth of chondrocytes for a few days in 5-bromo-2''-deoxyuridine, growth in this analog accelerates those changes that eventually lead to cellular senescence.