Chemo Proling and Simultaneous Analysis of Different Combinations of Sinomenii Caulis and Ramulus Cinnamomi Using UHPLC-Q-TOF-MS, GC-MS and HPLC Methods
Chemo Proling and Simultaneous Analysis of Different Combinations of Sinomenii Caulis and Ramulus Cinnamomi Using UHPLC-Q-TOF-MS, GC-MS and HPLC Methods
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DOI：
10.1093/chromsci/bmab048
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发表时间：
2021-05-10
影响因子：
1.3
通讯作者：
Gui, Shuangying
中科院分区：
文献类型：
--
作者：
Liu, Liu;Chu, Xiaoqin;Gui, Shuangying
目的：青风藤和桂枝是传统的抗炎中药。在本研究中，研究小组计划找出两种不同比例的中药组合的物质基础。方法：采用超高效液相色谱-四极杆飞行时间质谱（UHPLC-Q-TOF-MS）和气相色谱-质谱联用技术对乙醇提取的不同比例中药复方的提取物进行分析，鉴定其基本化学成分。同时采用高效液相色谱梯度洗脱法测定了中药配方中青藤碱、木兰花碱、月桂叶碱、蝙蝠葛碱、香豆素、肉桂醇、肉桂酸、肉桂醛等8种主要成分的含量。并采用等度洗脱法分别测定了青藤碱和肉桂醛的含量。结果：采用UHPLC-Q-TOF-MS对方剂中的18种化合物进行了鉴定。桂枝中的成分多为挥发油，以4:1配方中分离出的化合物种类最多。其中含量测定中确定了8个化合物为主要检测目标。结论：清风藤中青藤碱的提取率与药对中桂枝的比例有关。同时，不同比例青藤碱的添加也对桂枝中肉桂醛的提取产生一定的影响。此外，该系列方法还成功应用于清风藤桂枝汤不同样品中化学成分的同时测定。
Objective: Sinomenii Caulis (QingFengTeng) and Ramulus Cinnamomi (GuiZhi) are traditional Chinese drugs that have been used for anti-inflammation. In this study, the team plans to find out the material basis of a Chinese herb combination composed of the two herbs with different ratios.Methods: The extracts of the herbal compound with various ratios obtained from ethanol extraction were analyzed by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) and gas chromatography coupled mass spectrometry to identify the basic chemical compounds. Simultaneously, the contents of the eight main components (sinomenine, magnoflorine, laurifoline, dauricine, coumarin, cinnamyl alcohol, cinnamic acid and cinnamaldehyde) from herb formula were determined by gradient elution by high-performance liquid chromatography. Furthermore, the content of sinomenine and cinnamaldehyde were determined by isocratic elution, respectively.Results: Eighteen compounds in the herb formula were identified by UHPLC-Q-TOF-MS. The components in the GuiZhi are mostly volatile oils and the kinds of compounds isolated from the formula in the ratio of 4:1 were the most. Wherein eight compounds were identified as the main detection targets in the content determination.Conclusion: The extraction rate of sinomenine in QingFengTeng was related to the proportion of GuiZhi in the drug pairs. Synchronously, the addition of sinomenine in different proportions also had some influence on the extraction of cinnamaldehyde in GuiZhi. Furthermore, the series of methods was successfully applied to the simultaneous determination of chemical compounds in different samples of QingFengTeng-GuiZhi decoction.