Cultivable microflora of plaque from full denture bases and adjacent palatal mucosa.
Cultivable microflora of plaque from full denture bases and adjacent palatal mucosa.
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来自全口义齿基托和邻近腭粘膜的可培养菌斑微生物区系。
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
A. Geering
中科院分区：
文献类型：
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作者：
F. Gusberti;T. Gada;N. Lang;A. Geering
厌氧培养技术和快速生化鉴定试验通常用于牙周菌斑的分析牙菌斑样本取自义齿基托和相应的邻近粘膜。结果与这些或更全面的微生物调查进行了比较。44个样本来自12名患者的义齿基托和邻近的腭粘膜，戴上全口义齿。然后在还原转运液中采集标本，并在厌氧手套箱中处理。将样品铺在富集胰蛋白酶大豆琼脂（ETSA）上，并在需氧和厌氧条件下培养。此外，将菌斑样本接种在沙氏培养基上。义齿和腭部菌斑均以兼性菌为主。从腭粘膜中回收的样品中的球菌数量显著增加。义齿菌斑中杆较多。两种情况下均存在厌氧菌。在厌氧菌中，糖分解类杆菌的频率最高，而韦荣氏球菌属的物种存在的最大数量。ETSA和Sabouraud需氧培养培养基的酵母菌回收率相似。正如在其他微生物研究中发现的，白色念珠菌仅占总可培养植物群的一小部分。
Anaerobic cultural techniques and rapid biochemical identification tests normally employed for periodontal plaque were utilized for the analysis of plaque samples taken from denture bases and corresponding adjacent mucosa. Results were compared with those or more comprehensive microbiological investigations. Forty four samples were obtained from the denture bases and adjacent palatal mucosa of 12 patients wearing full upper dentures. The specimens were then collected in Reduced Transport Fluid and processed in the anaerobic glove box. Samples were plated on Enriched Trypticase Soy Agar (ETSA) and cultured under both aerobic and anaerobic conditions. In addition plaque samples were plated on Sabouraud medium. Facultative bacteria predominated in both denture and palatal plaques. Significantly greater numbers of cocci were recovered in the samples from palatal mucosa. More rods were found in denture plaque. Anaerobes were present in both instances. Among the anaerobes, saccharolytic Bacteroides were identified with the highest frequency, while Veillonella species were present in the greatest quantity. Recovery of yeasts was similar for both ETSA and Sabouraud media incubated aerobically. As has been found in other microbiological studies Candida albicans represented only a minor part of the total cultivable flora.