Combined Training Enhances Skeletal Muscle Mitochondrial Oxidative Capacity Independent of Age
Combined Training Enhances Skeletal Muscle Mitochondrial Oxidative Capacity Independent of Age
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DOI：
10.1210/jc.2014-3081
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发表时间：
2015-04-01
影响因子：
5.8
通讯作者：
Nair, K. Sreekumaran
中科院分区：
文献类型：
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作者：
Irving, Brian A.;Lanza, Ian R.;Nair, K. Sreekumaran
内容：久坐的老年人骨骼肌线粒体丰度和氧化能力降低。目的：主要目的是确定8周的综合训练（CT）是否比耐力训练（ET）或阻力训练（RT）对健康年轻人线粒体生理学有更强的影响干预：34名年轻人和31名老年人被随机分配到8周的ET、RT和对照/CT组。对照组受试者完成8周不运动（对照组），然后进行8周CT。在干预前后测量身体成分、骨骼肌强度和峰值摄氧量。在干预前和干预后48小时获得股外侧肌活检样本。线粒体生理学进行了评估，通过高分辨率呼吸和表达的线粒体蛋白和转录因子的定量PCR和immunoblotting.Results：ET和CT显着增加线粒体蛋白和转录因子的氧化能力和表达。所有的训练方式都改善了身体成分、心肺功能和骨骼肌力量。尽管ET和RT组件的训练量较低，但CT诱导了最稳健的脊髓相关结局和身体特征的改善。重要的是，大多数的适应训练发生独立的年龄.Conclusion：总的来说，这些结果表明，ET和CT增加肌肉线粒体丰度和能力，虽然CT引起最强大的改善测量的结果。总之，CT提供了一个强大的运动方案，以改善肌肉线粒体的结果和身体特征独立于年龄。
Context: Skeletal muscle from sedentary older adults exhibits reduced mitochondrial abundance and oxidative capacity.Objective: The primary objective was to determine whether 8 weeks of combined training (CT) has a more robust effect than endurance training (ET) or resistance training (RT) on mitochondrial physiology in healthy young (18-30 years) and older (>= 65 years) adults.Intervention: Thirty-four young and 31 older adults were randomly assigned to 8 weeks of ET, RT, and control/CT. Control subjects completed 8 weeks of no exercise (control) followed by 8 weeks of CT. Body composition, skeletal muscle strength, and peak oxygen uptake were measured before and after the intervention. Vastus lateralis muscle biopsy samples were obtained before and 48 hours after the intervention. Mitochondrial physiology was evaluated by high-resolution respirometry and expression of mitochondrial proteins and transcription factors by quantitative PCR and immunoblotting.Results: ET and CT significantly increased oxidative capacity and expression of mitochondrial proteins and transcription factors. All training modalities improved body composition, cardiorespiratory fitness, and skeletal muscle strength. CT induced the most robust improvements in mitochondria-related outcomes and physical characteristics despite lower training volumes for the ET and RT components. Importantly, most of the adaptations to training occurred independent of age.Conclusion: Collectively, these results demonstrate that both ET and CT increase muscle mitochondrial abundance and capacity although CT induced the most robust improvements in the outcomes measured. In conclusion, CT provides a robust exercise regimen to improve muscle mitochondrial outcomes and physical characteristics independent of age.