Generation of Porous Poly(Ethylene Glycol) Hydrogels by Salt Leaching
Generation of Porous Poly(Ethylene Glycol) Hydrogels by Salt Leaching
复制标题
DOI：
10.1089/ten.tec.2009.0646
复制
发表时间：
2010-10-01
影响因子：
3
通讯作者：
Brey, Eric M.
中科院分区：
文献类型：
--
作者：
Chiu, Yu-Chieh;Larson, Jeffery C.;Brey, Eric M.
聚（乙二醇）（PEG）水凝胶已被研究用于组织工程中的许多应用。水凝胶可以被设计成模拟具有所需化学和机械性质的组织，但它们的物理结构可能阻碍细胞迁移、组织侵入和分子运输。多孔PEG水凝胶的合成可以改善转运，增强细胞行为，并增加可用于细胞粘附的表面积。盐浸法已被广泛用于生产多孔生物材料支架，但以前没有应用于水凝胶。在这篇文章中，我们描述了一个修改传统的盐浸技术应用于水凝胶。制备盐饱和的聚合物前体溶液，并在聚合之前加入限定尺寸的盐晶体。然后盐晶体被滤出，产生多孔水凝胶。提供了将该技术应用于PEG水凝胶的实例。多孔PEG水凝胶产生的孔径范围从15至86 μ m和孔隙率从30%至75%。与细胞粘附肽结合的多孔水凝胶支持细胞粘附，其形态随孔径而变化。这里描述的简单的，可重复的技术可用于生成具有受控孔径的多孔水凝胶，用于组织工程。
Poly(ethylene glycol) (PEG) hydrogels have been investigated for a number of applications in tissue engineering. The hydrogels can be designed to mimic tissues that have desired chemical and mechanical properties, but their physical structure can hinder cell migration, tissue invasion, and molecular transport. Synthesis of porous PEG hydrogels could improve transport, enhance cell behavior, and increase the surface area available for cell adhesion. Salt leaching methods have been used extensively to generate porous biomaterial scaffolds but have not previously been applied to hydrogels. In this article we describe a modification of traditional salt leaching techniques for application to hydrogels. Salt-saturated polymer precursor solutions are prepared, and salt crystals of a defined size are added before polymerization. The salt crystals are then leached out, resulting in porous hydrogels. Examples are provided for application of this technique to PEG hydrogels. Porous PEG hydrogels were generated with pore sizes ranging from 15 to 86 mu m and porosities from 30% to 75%. Porous hydrogels that were incorporated with a cell adhesion peptide supported cell adhesion with morphology varying with pore size. The simple, reproducible technique described here could be used to generate porous hydrogels with controlled pore sizes for applications in tissue engineering.