TEMPORAL AND SPATIAL PATTERNS OF EXPRESSION OF C-FOS, ZIF-268, C-JUN AND JUN-B MESSENGERS RNAS IN RAT-BRAIN FOLLOWING SEIZURES EVOKED FOCALLY FROM THE DEEP PREPIRIFORM CORTEX
TEMPORAL AND SPATIAL PATTERNS OF EXPRESSION OF C-FOS, ZIF-268, C-JUN AND JUN-B MESSENGERS RNAS IN RAT-BRAIN FOLLOWING SEIZURES EVOKED FOCALLY FROM THE DEEP PREPIRIFORM CORTEX
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DOI：
10.1006/exnr.1993.1003
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发表时间：
1993-01-01
影响因子：
5.3
通讯作者：
GRAYSON, DR
中科院分区：
文献类型：
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作者：
LANAUD, P;MAGGIO, R;GRAYSON, DR
使用原位杂交，我们之前研究了 (17) 位于深前梨状皮层的高度限制性致癫痫部位引发惊厥性癫痫发作后，大脑中 c-fosmRNA 增加的区域模式。在本文中，我们重点关注海马体并检查编码其他立即早期基因 (IEG)（即 c-jun、jun-B 和 zif/268）的 mRNA，以了解局灶性癫痫发作后的变化。尽管每个 IEG mRNA 增加的解剖分布在定性上具有可比性，但时间分析表明 inzif/268 mRNA 的增加出现在研究的其他基因之前。每个 mRNA 在 30 分钟内达到最大增加，并在癫痫发作后 3 小时内下降至基础水平。数据表明，这四种 IEG 通过 mRNA 的增加以协调的方式对癫痫发作活动的传播做出反应，此外，所有四种基因的表达增加似乎发生在海马中相同的细胞类型中。
Usingin situhybridization, we previously investigated (17) the regional pattern of c-fosmRNA increases in the brain following convulsive seizures elicited from a highly circumscribed epileptogenic site located in the deep prepiriform cortex. In this paper, we focus on the hippocampus and examine mRNAs encoding other immediate early genes (IEGs), namely c-jun,jun-B, andzif/268, for changes following the focally evoked seizures. Although the anatomic distribution of increases in each IEG mRNA was qualitatively comparable, the temporal analysis indicated that increases inzif/268 mRNA appeared prior to the other genes studied. Each of the mRNAs reached a maximum increase by 30 min and declined to basal levels within 3 h following seizure initiation. The data indicate that these four IEGs respond in a coordinated fashion to propagated seizure activity with increases in mRNA and, furthermore, that increased expression of all four genes appears to occur in the same cell types in the hippocampus.