A new UV-LED device for automatic disinfection of stethoscope membranes
A new UV-LED device for automatic disinfection of stethoscope membranes
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DOI：
10.1016/j.ajic.2015.06.019
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发表时间：
2015-10-01
影响因子：
4.9
通讯作者：
Cevenini, Gabriele
中科院分区：
文献类型：
--
作者：
Messina, Gabriele;Burgassi, Sandra;Cevenini, Gabriele
背景：听诊器被医生和护士广泛使用。恶劣的听诊器卫生是医院感染的潜在来源。本研究旨在基于紫外线发光二极管(LED)的最新进展，提出一种用于听诊器膜自动高效消毒的创新解决方案。方法：将金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和粪肠球菌接种在28个听诊器膜上，然后转移到培养皿中。治疗包括用紫外线发光二极管照射暴露的皮氏培养皿1分钟。对于每种微生物，使用Wilcoxon符号等级测试比较了对照组和处理组在36摄氏度时的菌落形成单位(CFU)数量。Kruskal-Wallis试验被用来评估细菌减少的百分比。结果：紫外线照射后所有细菌的菌落总数均有显著下降(P<0.01)：粪肠球菌85.5%，金黄色葡萄球菌S 87.5%，大肠埃希菌94.3%，铜绿假单胞菌94.9%。不同细菌的CFU下降百分率无显著差异(P>；.01)。结论：听诊器是医学和保健专业人员的象征，已被证明是微生物的载体。该处理技术对膜的消毒是有效和高效的。这些有希望的结果代表着用一种创新的方法消除听诊器膜污染的一步。版权所有(C)2015年，感染控制和流行病学专业人士协会。Elsevier Inc.出版。保留所有权利。
Background: Stethoscopes are widely used by doctors and nurses. Poor stethoscope hygiene is a potential source of nosocomial infection. This study aimed to propose an innovative solution, based on the latest advances in ultraviolet (UV) light-emitting diodes (LEDs), for disinfecting stethoscope membranes automatically and efficiently.Methods: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Enterococcus faecalis were sown on 28 stethoscope membranes and then transferred to Petri dishes. Treatment involved illuminating exposed Petri dishes with a UVC LED for 1 minute. For each microbe, the number of colony-forming units (cfu) at 36 degrees C was compared in control and treated dishes using the Wilcoxon signed-rank test. The Kruskal-Wallis test was used to assess percent reductions in bacteria. Statistical significance was set at 99%.Results: A significant reduction in cfu counts after UV treatment (P < .01) was found for all bacteria: 85.5% for E faecalis, 87.5% for S aureus, 94.3% for E coli, and 94.9% for P aeruginosa. No significant differences in percent reduction in cfu were found between bacteria (P > .01).Conclusion: The stethoscope, symbol of medicine and health care professionals, has been demonstrated to be a carrier of microorganisms. The treatment technique was effective and efficient in disinfecting the membranes. These promising results represent a step forward toward eliminating stethoscope membrane contamination with an innovative approach. Copyright (C) 2015 by the Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.