Reactive oxygen species (ROS) reduce the expression of BRAK/CXCL14 in human head and neck squamous cell carcinoma cells
Reactive oxygen species (ROS) reduce the expression of BRAK/CXCL14 in human head and neck squamous cell carcinoma cells
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DOI：
10.3109/10715762.2010.490836
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发表时间：
2010-08-01
影响因子：
3.3
通讯作者：
Lee, Masaichi-Chang-Il
中科院分区：
文献类型：
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作者：
Maehata, Yojiro;Ozawa, Shigeyuki;Lee, Masaichi-Chang-Il
本研究探讨了由活性氧（ROS）诱导的氧化应激，如过氧化氢（H2 O2）和羟基自由基（HO中心点），对头颈部鳞状细胞癌（HNSCC）细胞中BRAK（也称为非ELR基序血管生成抑制CXC趋化因子配体14（CXCL 14））表达的影响。当HNSCC细胞在ROS存在下培养时，BRAS的表达显著降低，而IL-8的表达显著增加。有趣的是，HO中心点对HNSCC细胞中两种基因表达的影响比H2 O2大得多。用N-乙酰-L-半胱氨酸（NAG）、表皮生长因子受体（埃格）和丝裂原活化蛋白激酶（MAPK）抑制剂预处理可减弱ROS对BRAKE和IL-8表达的影响。这些结果表明，H2 O2或HO中心点诱导的氧化应激通过EGFR/MEK/ERK途径改变人HNSCC细胞中BRAKE和IL-8的基因表达，从而刺激血管生成和肿瘤进展。
The present study investigated the effects of oxidative stress induced by reactive oxygen species (ROS), such as hydrogen peroxide (H2O2) and hydroxyl radical (HO center dot), on the expression of both BRAK, which is also known as non-ELR motif angiostatic CXC chemokine ligand 14 (CXCL14), in head and neck squamous cell carcinoma (HNSCC) cells. When HNSCC cells were cultured in the presence of ROS, the expression of BRAK was significantly decreased whereas that of IL-8 was increased. Interestingly, the effects on the expression of both genes in HNSCC cells were much greater with HO center dot than with H2O2. The effects of ROS on both BRAK and IL-8 expression were attenuated by pre-treatment with N-acetyl-L-cysteine (NAG), epidermal growth factor receptor (EGER), and mitogen-activated protein kinase (MAPK) inhibitors. These results indicate that oxidative stress induced by H2O2 or HO center dot stimulates angiogenesis and tumuor progression by altering the gene expression of BRAK and IL-8 via the EGFR/MEK/ERK pathway in human HNSCC cells.