Identification of adenoid biofilms with middle ear pathogens in otitis-prone children utilizing SEM and FISH
Identification of adenoid biofilms with middle ear pathogens in otitis-prone children utilizing SEM and FISH
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DOI：
10.1016/j.ijporl.2009.05.016
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发表时间：
2009-09-01
影响因子：
1.5
通讯作者：
Coticchia, James M.
中科院分区：
文献类型：
--
作者：
Hoa, Michael;Tomovic, Senja;Coticchia, James M.
目的：生物膜与几种慢性感染的发生有关。我们试图证明中耳病原体在腺样体生物膜使用扫描电子显微镜（SEM）和荧光原位杂交（FISH）与共聚焦激光扫描显微镜（CLSM.Methods）：比较显微解剖调查腺样体粘膜使用SEM和FISH与共聚焦扫描激光显微镜（CLSM）成像从患者复发性急性中耳炎（RAOM）。所有中耳炎倾向的儿童表现出生物膜表面积的存在大于85%的扫描电镜。FISH伴随CLSM成像也表现出斑片状的生物膜所有生物膜中含有中耳病原体，并经常在多微生物分布：4 6，4 6和3 6个样本中含有流感嗜血杆菌，肺炎链球菌和卡他莫拉菌，分别。结论：致密的腺样体生物膜可以作为一个水库的tubotympanum再感染。在上呼吸道病毒感染过程中，存在于抗性腺样体生物膜中的嗜酸性中耳病原体的吸入可能是RAOM发展的重要事件。(c)2009爱思唯尔爱尔兰有限公司保留所有权利。
Objectives: Biofilms have been implicated in the development of several chronic infections. We sought to demonstrate middle ear pathogens in adenoid biofilms using scanning electron microscopy (SEM) and fluorescent in situ hybridization (FISH) with confocal laser scanning microscopy (CLSM).Methods: Comparative micro-anatomic investigation of adenoid mucosa using SEM and FISH with confocal scanning laser microscopic (CLSM) imaging from patients with recurrent acute otitis media (RAOM).Results: All otitis-prone children demonstrated biofilm surface area presence greater than 85% by SEM. FISH accompanied by CLSM imaging also demonstrated patchy biofilms All biofilms contained middle ear pathogens and were frequent in polymicrobial distributions: 4 of 6, 4 of 6 and 3 of 6 samples contained Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis, respectively.Conclusions: Dense adenoid biofilms may act as a reservoir for reinfection of the tubotympanum. Aspiration of planktonic middle ear pathogens existing in resistant adenoid biofilms during a viral upper respiratory tract infection may be an important event in the development of RAOM. (c) 2009 Elsevier Ireland Ltd. All rights reserved.