MRI detects white matter reorganization after neural progenitor cell treatment of stroke
MRI detects white matter reorganization after neural progenitor cell treatment of stroke
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DOI：
10.1016/j.neuroimage.2006.05.025
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发表时间：
2006-09-01
期刊：
影响因子：
5.7
通讯作者：
Chopp, Michael
中科院分区：
文献类型：
--
作者：
Jiang, Quan;Zhang, Zheng Gang;Chopp, Michael
我们利用MRI评估神经祖细胞治疗脑卒中对脑白质重组的影响。雄性Wistar大鼠（n = 26）大脑中动脉闭塞3 h后给予神经祖细胞治疗（n = 17）或不给予治疗（n = 9）， 5-7周后处死。MRI测量显示，移植的神经祖细胞选择性地向缺血边界区迁移。与对照组和治疗组相比，脑卒中后脑缺血恢复区脑白质重组与各向异性分数增加（FA, P < 0.01）一致。免疫反应染色显示脑卒中后神经元轴突突出，从胼胝体向同侧纹状体延伸。由扩散张量成像得出的纤维跟踪（FT）图显示了与免疫组织学结果相似的取向模式。脑卒中患者的补充测量表明，FT图显示出与病变边界平行的总体方向。我们的数据表明，FA和FT识别和表征脑卒中和神经祖细胞治疗后发生白质重组的脑组织。(c) 2006爱思唯尔公司版权所有。
We evaluated the effects of neural progenitor cell treatment of stroke on white matter reorganization using MRI. Male Wistar rats (n = 26) were subjected to 3 h of middle cerebral artery occlusion and were treated with neural progenitor cells (n = 17) or without treatment (n = 9) and were sacrificed at 5-7 weeks thereafter. MRI measurements revealed that grafted neural progenitor cells selectively migrated towards the ischemic boundary regions. White matter reorganization confirmed histologically, was coincident with increases of fractional anisotropy (FA, P < 0.01) after stroke in the ischemic recovery regions compared to that in the ischemic core region in both treated and control groups. Immunoreactive staining showed axonal projections emanating from neurons and extruding from the corpus callosum into the ipsilateral striatum bounding the lesion areas after stroke. Fiber tracking (FT) maps derived from diffusion tensor imaging revealed similar orientation patterns to the immunohistological results. Complementary measurements in stroke patients indicated that FT maps exhibit an overall orientation parallel to the lesion boundary. Our data demonstrate that FA and FT identify and characterize cerebral tissue undergoing white matter reorganization after stroke and treatment with neural progenitor cells. (c) 2006 Elsevier Inc. All rights reserved.