GENOME SIZE OF MYXOCOCCUS-XANTHUS DETERMINED BY PULSED-FIELD GEL-ELECTROPHORESIS
GENOME SIZE OF MYXOCOCCUS-XANTHUS DETERMINED BY PULSED-FIELD GEL-ELECTROPHORESIS
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DOI：
10.1128/jb.172.8.4206-4213.1990
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发表时间：
1990-08-01
影响因子：
3.2
通讯作者：
SHIMKETS, LJ
中科院分区：
文献类型：
--
作者：
CHEN, HW;KESELER, IM;SHIMKETS, LJ
用稀有的限制性内切酶AseI或Spei对黄色粘细菌基因组DNA进行酶切，并用脉冲场凝胶电泳法分离限制性内切酶产物。利用含有AseI酶切位点的转座子Tn5-132和Tn5lac测定每条带的限制性片段数。通过添加限制性内切酶产物的分子大小来确定基因组的大小。DK101、MD2和DZF1的基因组具有相同的限制性内切酶图谱，估计为9,454.+-。来自东盟文摘的101千碱基对和9453.+-。来自SPI摘要的106千碱基对。DK1622是从DK101经紫外光处理而来的，它经历了220至222千碱基的缺失，移除了AseI和SpeI限制位点。缺失的DNA可能完全由Mxα相关序列组成。
Genomic DNA of the myxobacterium Myxococcus xanthus was digested with the rare cutting restriction endonuclease AseI or SpeI, and the restriction products were separated by pulsed-field gel electrophoresis. Transposons Tn5-132 and Tn5 lac, which contain AseI restriction sites, were used to determine the number of restriction fragments in each band. The size of the genome was determined by adding the molecular sizes of the restriction products. The genomes of strains DK101, MD2, and DZF1 have identical restriction patterns and were estimated to be 9,454 .+-. 101 kilobase pairs from the AseI digestions and 9,453 .+-. 106 kilobase pairs from the SpeI digestions. DK1622, which was derived from DK101 by treatment with UV light, has suffered a 220- to 222-kilobase-pair deletion that removed an AseI and an SpeI restriction site. The deleted DNA may consist exclusively of Mx alpha-associated sequences.