Effect of gelation conditions on the gel structure and resolving power of agarose‐based DNA sequencing gels
Effect of gelation conditions on the gel structure and resolving power of agarose‐based DNA sequencing gels
复制标题
DOI：
10.1002/(sici)1522-2683(19990601)20:7
复制
发表时间：
1999-06
期刊：
影响因子：
2.9
通讯作者：
N. Kusukawa;M. Ostrovsky;M. Garner
中科院分区：
文献类型：
--
作者：
N. Kusukawa;M. Ostrovsky;M. Garner
研究了凝胶化条件对琼脂糖基DNA测序凝胶结构和分辨性能的影响。通过快速冷却形成的凝胶具有清晰、清晰的条带分离模式，并且在允许缓慢冷却的凝胶上不分离的碱基重复序列被分解。电镜分析表明，快速冷却的凝胶具有均匀的孔隙结构，平均孔径小于缓慢冷却的凝胶。这两种类型的凝胶在光学清晰度上也不同，并且通过差示扫描量热法测量其熔化特性有显着差异。
The effect of gelation conditions on the structure and resolving properties of agarose‐based DNA sequencing gels were studied. Gels that were formed by rapid cooling gave separation patterns with sharp, well‐defined bands, and resolved base repeats that were not separated on gels which were allowed to cool slowly. Analyses by electron microscopy indicated that the rapidly cooled gels have a homogeneous pore structure with a smaller average pore size than gels that were cooled slowly. The two types of gels also differ in optical clarity, and have significant differences in their melting characteristics as measured by differential scanning calorimetry.