Pathogenicity of H5 influenza viruses for ducks
Pathogenicity of H5 influenza viruses for ducks
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DOI：
10.1007/s00705-004-0473-x
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发表时间：
2005-07-01
影响因子：
2.7
通讯作者：
Kida, H
中科院分区：
文献类型：
--
作者：
Kishida, N;Sakoda, Y;Kida, H
对4株H5 N1亚型高致病性禽流感病毒和1株弱毒株H5 N1亚型禽流感病毒在家鸭体内的致病性进行了测定。在日本高致病性禽流感爆发期间从死禽中分离的A/chicken/Yamaguchi/7/04（H5 N1）（Ck/Yamaguchi/04）和在从中国进口的检疫检查中从鸭肉中分离的A/duck/Yokohama/aq-10/03（H5 N1）（Dk/Yokohama/03）在包括鸭脑在内的多个器官中复制。感染Ck/Yamaguchi/04的鸭没有表现出任何临床症状，而感染Dk/Yokohama/03的鸭表现出神经系统症状。感染A/Hong Kong/483/97（H5 N1）或A/tern/South Africa/61（H5 N3）或无毒H5 N1疫苗的鸭子均未出现任何临床症状。病毒特异性抗体的检测与每一个测试的五个菌株感染的鸭的血清中，表明所有的病毒株感染和复制的鸟类。Dk/Yokohama/03在多个器官中的生长速度比Ck/Yamaguchi/04快。在感染Dk/Yokohama/03的鸭的脑中检测到相当大滴度的病毒，这些鸟表现出神经系统症状。目前的结果表明，鸭流感病毒的致病性与鸡没有相关性，病毒在脑中的复制是鸭表现出神经系统症状的关键。
Four H5N1 highly pathogenic avian influenza (HPAI) viruses and an avirulent reassortant H5N1 virus were tested for their pathogenicity in domestic ducks. A/chicken/Yamaguchi/7/04 (H5N1) (Ck/Yamaguchi/04) isolated from a dead bird during the HPAI outbreak in Japan and A/duck/Yokohama/aq-10/03 (H5N1) (Dk/Yokohama/03) isolated from duck meat at a quarantine inspection for importation from China replicated in multiple organs including the brain of ducks. The ducks infected with Ck/Yamaguchi/04 did not show any clinical signs, while those infected with Dk/Yokohama/03 showed neurological signs. The ducks infected either with A/Hong Kong/483/97 (H5N1) orA/tern/SouthAfrica/61 (H5N3), or with an avirulent H5N1 reassortant, did not show any clinical signs. Virus-specific antibodies were detected in the sera of the ducks infected with each of the five strains tested, indicating that all of the viral strains infected and replicated in the birds. Dk/Yokohama/03 grew in multiple organs more rapidly than did Ck/Yamaguchi/04. Considerable titers of virus were detected in the brain of the ducks infected with Dk/Yokohama/03 and these birds showed neurological signs. The present results demonstrate that the pathogenicity of influenza viruses for ducks does not correlate with that for chickens and that replication of the virus in the brain is critical for ducks to show neurological signs.