Dihydropyridine receptor gene expression in skeletal muscle from mdx and control mice.
Dihydropyridine receptor gene expression in skeletal muscle from mdx and control mice.
复制标题
mdx 和对照小鼠骨骼肌中二氢吡啶受体基因的表达。
DOI：
10.1016/s0925-4439(97)00079-3
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Palade,PT
中科院分区：
文献类型：
--
作者：
Péréon,Y;Dettbarn,C;Navarro,J;Noireaud,J;Palade,PT
研究了异型特异性二氢吡啶受体钙通道(DHPR) α1亚基基因在mdx和对照小鼠膈肌（DIA）和前胫肌（TA）中的表达。采用骨骼肌特异性大鼠DHPR cDNA探针和心肌特异性小鼠DHPR cDNA探针进行RNase保护实验。在对照组和mdx小鼠的TA肌中，编码心脏DHPR的基因表达水平都很弱。与TA相比，DIA表达的心脏异构体mRNA水平显著高于TA，但mdx和对照小鼠DIA水平相似。相比之下，DHPR骨骼肌亚型的mRNA表达水平在对照DIA中低于TA。然而，与对照组相比，mdx DIA中DHPR骨骼肌亚型的表达显著增加，达到TA表达水平，而营养不良不影响TA表达。[3H]-PN200-110结合进一步评估DIA DHPR在蛋白水平上的表达。与对照组小鼠相比，mdx的DIA肌肉中探针结合位点的密度没有明显影响，但在老年mdx和对照组小鼠中有所降低。DHPR mRNA水平升高而DHPR蛋白表达未随之升高可能是由于mdx DIA中可能发生的蛋白质降解增强所致。这里发现的DHPR表达水平的改变似乎并不是mdx DIA收缩功能严重缺陷的原因。
The expression of isoform-specific dihydropyrine receptor–calcium channel (DHPR) α1-subunit genes was investigated in mdx and control mouse diaphragm (DIA) and tibialis anterior (TA). RNase protection assays were carried out with a rat DHPR cDNA probe specific for skeletal muscle and a mouse DHPR cDNA probe specific for cardiac muscle. The level of expression of the gene encoding the cardiac DHPR was very weak in TA muscle from both control and mdx mice. Compared to TA, DIA expressed mRNA for the cardiac isoform at significantly higher levels, but mdx and control mouse DIA levels were similar to one another. In contrast, mRNA expression levels for the DHPR skeletal muscle isoform were lower in control DIA than TA. However, there was a dramatic increase in the expression for the DHPR skeletal muscle isoform in mdx DIA compared with control DIA, reaching the TA expression level, whereas dystrophy did not affect TA expression. [3H ]-PN200-110 binding was used to further assess DIA DHPR expression at the protein level. The density of binding sites for the probe was not significantly affected in DIA muscles of mdx vs. control mice, but it was reduced in older mdx and control mice. The increase in DHPR mRNA levels without a consequent increase in DHPR protein expression could be secondary to possible enhanced protein degradation which occurs in mdx DIA. The altered DHPR expression levels found here do not appear to be responsible for the severe deficits in contractile function of the mdx DIA.
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DOI：
10.1016/0005-2760(72)90034-3
发表时间：
1972-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
BILHEIMER, DW;LEVY, RI;EISENBERG, S
通讯作者：
EISENBERG, S
高甘油三酯血症和多分散低密度脂蛋白受试者中载脂蛋白 B 的代谢。
影响因子：
6.5
作者：
W. Fisher;L. Zech;P. Bardalaye;G. Warmke;M. Berman
通讯作者：
M. Berman
卵磷脂：胆固醇酰基转移酶诱导的非洲绿猴肝灌注液盘状高密度脂蛋白的修饰。
影响因子：
6.5
作者：
Babiak,J;Tamachi,H;Johnson,FL;Parks,JS;Rudel,LL
通讯作者：
Rudel,LL
人类 apoB 和 apoC 脂蛋白的代谢：正常和高脂蛋白血症受试者的动力学研究。
影响因子：
6.5
作者：
M. Berman;M. Hall;R. Levy;S. Eisenberg;D. Bilheimer;R. Phair;R. Goebel
通讯作者：
R. Goebel