Attaching proteins to carbon nanotubes via diimide-activated amidation
Attaching proteins to carbon nanotubes via diimide-activated amidation
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DOI：
10.1021/n1010095i
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发表时间：
2002-04-01
期刊：
影响因子：
10.8
通讯作者：
Sun, YP
中科院分区：
文献类型：
--
作者：
Huang, WJ;Taylor, S;Sun, YP
Carbon nanotubes are functionalized by bovine serum albumin (BSA) proteins via diimide-activated amidation under ambient conditions. The nanotube-BSA conjugates thus obtained are highly water-soluble, forming dark-colored aqueous solutions. Results from characterizations using atomic force microscopy (AFM), thermal gravimetric analysis, Raman, and gel electrophoresis show that the conjugate samples indeed contain both carbon nanotubes and BSA proteins and that the protein species are intimately associated with the nanotubes. Bioactivities of the nanotube-bound proteins are evaluated using the total protein micro-determination assay (the modified Lowry procedure). The results show that the overwhelming majority (similar to90%) of the protein species in the nanotube-BSA conjugates remain bioactive.
