Elevated serum level of lncRNA-HIF1A-AS1 as a novel diagnostic predictor for worse prognosis in colorectal carcinoma
Elevated serum level of lncRNA-HIF1A-AS1 as a novel diagnostic predictor for worse prognosis in colorectal carcinoma
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DOI：
10.3233/cbm-170179
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发表时间：
2017-01-01
影响因子：
3.1
通讯作者：
Yang, Zhenhua
中科院分区：
文献类型：
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作者：
Gong, Wanjun;Tian, Ming;Yang, Zhenhua
探讨血清长链非编码RNA（lncRNA）HIF 1 α反义RNA 1（HIF 1A-AS 1）对结直肠癌（CRC）的诊断价值及预后价值。我们从151名CRC患者和160名健康对照中获得血清样本。采用实时荧光定量PCR（RT-PCR）检测血清HIF 1A-AS 1水平。应用受试者工作特征（ROC）曲线分析HIF 1A-AS 1的诊断价值。将大肠癌组织分为HIF 1A-AS 1高表达组和HIF 1A-AS 1低表达组，分析HIF 1A-AS 1血清水平与大肠癌临床病理特征及预后的关系。大肠癌患者血清HIF 1A-AS 1水平显著高于正常对照组（P < 0.05）。ROC曲线分析显示，HIF 1A-AS 1在区分CRC和健康对照方面具有相对较高的诊断性能，曲线下面积（AUC）为0.960（95%CI：0.940与0.980相似; P < 0.001）。Kaplan-Meier分析显示分化程度、肿瘤大小、TNM分期、T分期、N分期、M分期及血清HIF 1A-AS 1水平均与大肠癌预后相关（P均< 0.05）。与HIF 1A-AS 1低表达的结直肠癌患者相比，HIF 1A-AS 1高表达的结直肠癌患者5年生存率较低（P < 0.001）。多因素考克斯回归分析显示，低分化、肿瘤> 5 cm、HIF 1A-AS 1高表达是影响大肠癌患者生存率的独立危险因素（P < 0.05）。提示HIF 1AAS 1可作为大肠癌诊断和预后的潜在生物标志物。
To evaluate the diagnostic efficacy and prognostic value of serum long non-coding RNA (lncRNA) HIF 1alpha-antisense RNA 1 (HIF1A-AS1) in patients with colorectal carcinoma (CRC). We obtained serum samples from 151 CRC patients and 160 health controls. Serum level of HIF1A-AS1 was detected via real-time PCR (RT-PCR). Receiver operating characteristics (ROC) curve analysis was conducted to determine the diagnostic value of HIF1A-AS1. Then HIF1A-AS1 in CRC was divided into high-and low-expression groups, and the associations of the HIF1A-AS1 serum level with clinicopathological features and prognosis were analyzed. Serum level of HIF1A-AS1 was significantly increased from CRC patients as compared to those of health controls (P < 0.05). ROC curve analysis revealed a relative high diagnostic performance of HIF1A-AS1 to distinguish CRC from health controls, with the area under the curves (AUC) of 0.960 (95% CI: 0.940 similar to 0.980; P < 0.001). Kaplan-Meier analysis showed that differentiation degree, tumor size, TNM stage, T stage, N stage, M stage and serum level of HIF1A-AS1 were all linked to CRC prognosis (All P < 0.05). Compared to CRC patients with low HIF1A-AS1 expression, high expression of patients were associated with a shorter 5-year-survival rate (P < 0.001). Multivariate Cox regression analysis revealed that lower differentiation degree, tumor > 5 cm and higher expression of HIF1A-AS1 were independent risk factors affecting the survival rate of patients with CRC (P < 0.05). Our results illustrated that elevated serum HIF1AAS1 could be clinically functioned as a potential biomarker for CRC diagnoses and prognosis.