In vivo imaging of chikungunya virus in mice and Aedes mosquitoes using a Renilla luciferase clone.
In vivo imaging of chikungunya virus in mice and Aedes mosquitoes using a Renilla luciferase clone.
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DOI：
10.1089/vbz.2011.0648
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发表时间：
2011-11
期刊：
影响因子：
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通讯作者：
S. Ziegler;J. Nuckols;C. Mcgee;Yan-Jang S. Huang;D. Vanlandingham;R. Tesh;S. Higgs
中科院分区：
文献类型：
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作者：
S. Ziegler;J. Nuckols;C. Mcgee;Yan-Jang S. Huang;D. Vanlandingham;R. Tesh;S. Higgs
基孔肯雅病毒（CHIKV）是一种蚊媒甲病毒，在人类中引起急性发热性疾病，其特征为发热、关节痛和皮疹。目前，它与亚洲、非洲和印度洋岛屿的大规模疫情有关，并已从这些热带地区传入欧洲，在欧洲已记录了两次局部传播。CHIKV和产生类似症状的相关甲病毒的发病机制的潜在基础仍不清楚。通过应用新技术，例如，活动物和节肢动物的体内成像，我们可以提高我们对脊椎动物中病毒发病机制和蚊子中病毒复制的理解。本技术报告描述了CHIKV？荧光素酶克隆以可视化埃及伊蚊和白纹伊蚊蚊子和小鼠中的感染和传播。在蚊子中，荧光素酶活性在感染后3天和7天在头部和头部都被观察到。在感染后长达5天，在小鼠中在接种部位检测到CHIKV-荧光素酶的体内成像，其中有限的传播到骨骼肌。
Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that in humans causes an acute febrile illness characterized by fever, arthralgia, and rash. It is currently associated with large outbreaks in Asia, Africa, and islands of the Indian Ocean and has been introduced from these tropical regions into Europe, where local transmission has been recorded on two occasions. The underlying basis of the pathogenesis of CHIKV and related alphaviruses that produce similar symptoms remains unclear. By applying new techniques, for example, in vivo imaging in live animals and arthropods, we may improve our understanding of viral pathogenesis in vertebrates and viral replication in mosquitoes. This technical report describes the evaluation of a CHIKV?luciferase clone to visualize infection and dissemination in both Aedes aegypti and Aedes albopictus mosquitoes and mice. In mosquitoes, luciferase activity was seen at 3 and 7 days post-infection in both head and abdomens. In vivo imaging of CHIKV-luciferase was detected in mice for up to 5 days post-infection at the site of inoculation with limited dissemination to the skeletal muscle.