Exposure to organophosphate pesticides and male hormone profile in floriculturist of the state of Morelos, Mexico
Exposure to organophosphate pesticides and male hormone profile in floriculturist of the state of Morelos, Mexico
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DOI：
10.1093/humrep/deq082
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发表时间：
2010-07-01
影响因子：
6.1
通讯作者：
Cebrian, Mariano E.
中科院分区：
文献类型：
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作者：
Blanco-Munoz, Julia;Mayanin Morales, Magally;Cebrian, Mariano E.
背景技术背景：对实验动物的研究发现，有机磷（OP）农药可能作为内分泌干扰物;然而，它们对人类激素的影响尚未得到充分的表征。我们评估暴露于OP农药之间的关联，通过二烷基磷酸盐（DAP）代谢产物尿水平，和男性激素profile.METHODS：一个横断面研究进行了104花卉种植者的莫雷洛斯，墨西哥。采用结构式问卷调查，以获得有关社会人口学特征，人体测量学，临床病史，酒精和烟草消费，以及工作史的信息。采用气液色谱法测定DAP代谢物[磷酸二甲酯（DMP）、硫代磷酸二甲酯、二硫代磷酸二甲酯、磷酸二乙酯（DEP）、硫代磷酸二乙酯（DETP）和二硫代磷酸二乙酯]。采用酶联免疫吸附法测定血清FSH、LH、催乳素、睾酮、雌二醇和卵泡抑素B水平。多元线性回归用于研究DAP代谢物水平和男性激素谱之间的关联。结果：在β，β '-二氯二苯二氯乙烯血清水平和其他潜在的混杂因素的影响下，β-二氯二苯二氯乙烯的代谢产物β-二氯二苯酯B与尿中β-二氯二苯酯、DEP、DETP和总DAP代谢产物的水平呈负相关。DEP水平与血清FSH浓度呈负相关，但与睾酮浓度呈轻微正相关。DETP与低LH血清水平轻微相关。OP代谢产物和血清激素水平之间没有其他显着的关联。结论：抑制素B和FSH根据职业暴露于OP农药的男性DAP代谢产物的水平而变化。这些结果表明，有机磷农药可以作为人类的内分泌干扰物;然而，大多数激素值都在正常范围内，相关性很小。因此，需要进一步研究，以阐明其生物学和临床相关性。
BACKGROUND: Studies on experimental animals have found that organophosphate (OP) pesticides may act as endocrine disruptors; however, their effects on the human hormonal profile have not yet been adequately characterized. We evaluate the association between exposure to OP pesticides, measured through dialkyl phosphate (DAP) metabolites urinary levels, and the male hormone profile.METHODS: A cross-sectional study was performed in 104 floriculturists of Morelos, Mexico. A structured questionnaire was applied to get information on sociodemographic characteristics, anthropometry, clinical history, alcohol and tobacco consumption, and work history. DAP metabolites [dimethylphosphate (DMP), dimethylthiophosphate, dimethyldithiophosphate, diethylphosphate (DEP), diethylthiophosphate (DETP) and diethyldithiophosphate] were determined using gas-liquid chromatography. Serum levels of FSH, LH, prolactin, testosterone, inhibin B and estradiol were determined using enzyme-linked immunosorbent assay. Multiple linear regression was used to study the association between DAP metabolite levels and male hormonal profile. Data were adjusted by p,p'-dichlorodiphenyldichloroethene serum levels and other potential confounders.RESULTS: There was a negative association between inhibin B and urinary levels of DMP, DEP, DETP and total DAP metabolites. DEP levels were negatively associated with serum FSH concentrations, but marginally and positively associated with those of testosterone. DETP was marginally associated with lower LH serum levels. There were no other significant associations among OP metabolites and serum hormone levels.CONCLUSIONS: Inhibin B and FSH vary according to levels of DAP metabolites in men occupationally exposed to OP pesticides. These results suggest that OP pesticides could act as endocrine disruptors in humans; however, most hormonal values fell within the wide normal range and associations were small. There is, therefore, a need for further investigation to elucidate their biological and clinical relevance.