Plasmid-mediated quinolone resistance and β-lactamases in Escherichia coli from healthy animals from Nigeria
Plasmid-mediated quinolone resistance and β-lactamases in Escherichia coli from healthy animals from Nigeria
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DOI：
10.1093/jac/dkr085
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发表时间：
2011-06-01
影响因子：
5.2
通讯作者：
Carattoli, Alessandra
中科院分区：
文献类型：
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作者：
Fortini, Daniela;Fashae, Kayode;Carattoli, Alessandra
目的：质粒介导的喹诺酮类耐药（PMQR）和β-内酰胺酶的动物储库仍有争议，关于这些耐药决定因素在发展中国家的流行情况的信息很少。本研究的目的是确定和表征PMQR和β-内酰胺酶的收集，从健康的鸡和猪在屠宰，收集在2006年11月至12月，在尼日利亚伊巴丹，氨苄青霉素耐药大肠杆菌分离。大肠杆菌从尼日利亚伊巴丹屠宰的健康鸡和猪中获得。采用纸片扩散法检测菌株的抗菌药物敏感性。Etest法测定环丙沙星的MIC。通过PCR和DNA测序筛选抗性基因。肠细菌重复基因间一致性PCR确定菌株的克隆相关性。结果：在18株E.其中11株对环丙沙星敏感性降低。12个菌株携带qnrS 1，3个菌株携带qnrB 19，1个菌株携带qnrB 10，3个菌株携带qepA; 1个菌株同时携带qepA和qnrB 10。所有菌株携带的bla（TEM）基因;一个菌株是阳性的CTX-M-15超广谱β-lactamase.Conclusions：我们的研究结果表明，食用动物可以代表一个重要的水库PMQR在非洲这一地区。先前的研究报告了尼日利亚人类临床分离株中qnr基因的高流行率，表明这些耐药决定因素在该国的传播可能特别相关。
Objectives: The animal reservoir of plasmid-mediated quinolone resistance (PMQR) and beta-lactamases is still controversial and little information is available on the prevalence of these resistance determinants in developing countries. The aim of this study was to identify and characterize PMQR and beta-lactamases in a collection of commensal ampicillin-resistant Escherichia coli isolated from healthy chickens and pigs at slaughter, collected in November-December 2006, in Ibadan, Nigeria.Methods: One hundred and sixty-two ampicillin-resistant E. coli were obtained from healthy chickens and pigs at slaughter in Ibadan, Nigeria. Strains were tested for antimicrobial susceptibility by disc diffusion assay. MICs of ciprofloxacin were determined by Etest. Resistance genes were screened by PCR and DNA sequencing. Clonal relatedness of the isolates was determined by enterobacterial repetitive intergenic consensus-PCR. Plasmids were transferred by conjugation and transformation and characterized by PCR-based replicon typing and plasmid multilocus sequence typing.Results: PMQR genes were detected in 18 E. coli strains; 11 of them showed reduced susceptibility to ciprofloxacin. Twelve strains carried qnrS1, three strains carried qnrB19, one strain carried qnrB10 and three strains carried qepA; one strain carried both qepA and qnrB10. All strains carried the bla(TEM) gene; one strain was positive for the CTX-M-15 extended-spectrum beta-lactamase.Conclusions: Our findings suggest that food animals could represent an important reservoir of PMQR in this region of Africa. Previous studies reported high prevalence of qnr genes in clinical isolates from humans in Nigeria, suggesting that the spread of these resistance determinants in this country could be particularly relevant.