Polyclonal antibody that recognizes calcium-dependent determinants in Tetrahymena calmodulin.
Polyclonal antibody that recognizes calcium-dependent determinants in Tetrahymena calmodulin.
复制标题
识别四膜虫钙调蛋白中钙依赖性决定簇的多克隆抗体。
DOI：
10.1021/bi00320a011
复制
发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Vanaman,TC
中科院分区：
文献类型：
--
作者：
McCartney,JE;Blum,JJ;Vanaman,TC
John E. McCartney, Jacob J. Blum, and Thomas C. Vanaman** abstract: We report here that a precipitating antibody prepared against Tetrahymena pyriformis calmodulin recognizes calcium-dependent determinants in the native protein. The ability of the antibody to precipitate 35S-labeled Tet-rahymena calmodulin in direct radioimmunoassays was en-hanced at least 3-fold in the presence of calcium. Competitive radioimmunoassay using homogeneous preparations of en-dogenously 35S-labeled Tetrahymena calmodulin and protein A-Sepharose-purified immunoglobulin G demonstrated that this antibody preparation is specific for protozoan calmodulin.
