MORPHOLOGY OF ABALONE SPERMATOZOA BEFORE AND AFTER THE ACROSOME REACTION
MORPHOLOGY OF ABALONE SPERMATOZOA BEFORE AND AFTER THE ACROSOME REACTION
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DOI：
10.1016/s0022-5320(80)90133-1
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
VACQUIER, VD
中科院分区：
文献类型：
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作者：
LEWIS, CA;LEIGHTON, DL;VACQUIER, VD
红鲍的精子，H. rufescens，由子弹形顶体颗粒（2.5 × 1000）组成。1.1μ m）、核（4.2 × 4.5 μ m）、1.0μ m）、中段（0.75 × 0.75 μ m）、中间段（0.75 × 0.75 μ m）。1.3由5个线粒体、一对中心粒和一系列囊泡以及鞭毛（45 × 106 μ m）组成。0.25μ m）。大的顶体颗粒含有2种不同的成分：在颗粒的前顶点中的卵圆形电子致密体（约0.9 × 104）; 1.0μ m）和在颗粒后部的较低密度的均匀材料。一束预先形成的肌动蛋白丝（长度约2.4 μ m）从位于前部的核窝突出到位于顶体颗粒后部的顶体窝中。海水中30 mM的Ca ~（2+）、离子载体A23187或精子老化都能触发鲍精子的顶体反应。在反应时，顶体颗粒释放水溶性组分（被认为是颗粒的电子密度较低的后部物质，留下壁厚约0.03 μ m的幽灵样空囊）。肌动蛋白为核心的顶体突起的延伸发生在顶体颗粒的胞吐作用之后。该方法（长度为7 μ m × 1000 μ m）是在100 ℃下进行的。0.2宽度为1 μ m）延伸穿过顶体颗粒的鞘状影。虚影的内表面布满小球（直径约0.04 μ m）。顶体突起的外表面覆盖着致密的物质，这些物质可能在精卵粘附中起作用。鲍鱼精子是精子形态的原始类型。
Spermatozoa of the red abalone, H. rufescens, consist of a bullet-shaped acrosome granule (2.5 .times. 1.1 .mu.m), a nucleus (4.2 .times. 1.0 .mu.m), a midpiece (0.75 .times. 1.3 .mu.m) consisting of 5 mitochondria, a pair of centrioles and a series of vesicles and a flagellum (45 .times. 0.25 .mu.m). The large acrosome granule contains 2 distinct components: an ovoid electron-dense body in the anterior apex of the granule (approximately 0.9 .times. 1.0 .mu.m) and a less dense homogeneous material at the granule posterior. A bundle of preformed actin filaments (about 2.4 .mu.m in length) protrude from the anteriorly located nuclear fossa into the acrosomal fossa located at the posterior of the acrosome granule. The acrosome reaction of Haliotis sperm can be triggered by 30 mM Ca2+ in the seawater, the ionophore A23187 or the aging of sperm. Upon reaction, the acrosome granule releases water-soluble components (thought to be the less electron-dense, posterior material of the granule, leaving a ghost-like empty sac with a wall approximately 0.03 .mu.m in thickness). The extension of the actin-cored acrosome process occurs immediately after the exocytosis of the acrosome granule. The process (7 .mu.m in length .times. 0.2 .mu.m in width) extends through the sheath-like ghost of the acrosome granule. The inner surface of the ghost is studded with globules (about 0.04 .mu.m diameter). The outer surface of the acrosome process is coated with dense material which may function in sperm-egg adhesion. Abalone sperm are of the primitive type of sperm morphology.