[A serum metabolomics study of gastric cancer based on pseudotargeted liquid chromatography-mass spectrometry approach].
[A serum metabolomics study of gastric cancer based on pseudotargeted liquid chromatography-mass spectrometry approach].
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基于伪靶向液相色谱-质谱法的胃癌血清代谢组学研究
DOI：
10.3724/sp.j.1123.2013.11050
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Guowang Xu
中科院分区：
文献类型：
--
作者：
Taizhong Yang;Ping Luo;Yanli Li;Rui Hua;Peiyuan Yin;Guowang Xu
胃癌是世界上最常见的恶性肿瘤之一，也是世界上第二大癌症相关死亡原因。包括肿瘤标志物在内的非侵入性筛查方法在临床上是迫切需要的。为寻找胃癌的代谢标志物，采用液相色谱-质谱联用技术（LC-MS），采用假靶向方法，对20例胃癌患者和40例健康对照者的血清代谢特征进行了分析。主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）结果表明，GC与健康对照组之间存在明显的分类。根据非参数统计检验的结果，在所有差异代谢特征中鉴定出57种离子。在所有鉴定的差异代谢物中，二氢胆固醇提供了良好的GC诊断性能，这也被另一个包括20名胃癌患者和39名健康对照的队列验证。通过这种强大的假靶向方法，我们的研究提供了潜在的肿瘤标志物和胃癌代谢表型的基础。
Gastric cancer is one of the most common malignant and the second most frequent cause of cancer-related mortality in the world. Noninvasive screen methods including tumor markers are intensively needed in the clinic. To discover metabolic markers for gastric cancers, the sera metabolic profiles of 20 gastric cancer (GC) patients and 40 healthy controls were analyzed by liquid chromatography coupled with mass spectrometry (LC-MS) using a pseudotargeted approach. The results of principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) indicated that obvious classification could be observed between GC and the healthy controls. According to the results of non-parametric statistical test, 57 ions were identified among all the differential metabolic features. Of all the identified differential metabolites, dihydrocholesterol provide good diagnostic performance of GC, which was also validated by another cohort including the 20 gastric cancer patients and the 39 healthy controls. With this robust pseudotargeted approach, our study provides both potential tumor markers and the basis for the metabolic phenotype of gastric cancers.