The twin arginine translocation system is essential for virulence of Yersinia pseudotuberculosis
The twin arginine translocation system is essential for virulence of Yersinia pseudotuberculosis
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DOI：
10.1128/iai.74.3.1768-1776.2006
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发表时间：
2006-03-01
影响因子：
3.1
通讯作者：
Forsberg, Å
中科院分区：
文献类型：
--
作者：
Lavander, M;Ericsson, SK;Forsberg, Å
对人类致病的耶尔森氏菌的 III 型分泌及其在毒力中的重要作用已被广泛表征。这项研究涉及革兰氏阴性细菌利用双精氨酸易位 (Tat) 途径将折叠蛋白穿过细菌内膜分泌到周质室中。我们已经证明，耶尔森氏菌 Tat 系统具有功能性，是运动所必需的，并且有助于抵抗酸性。然而，具有破坏的 Tat 系统 (tatC) 的假结核耶尔森氏菌突变株在体外生长中未受到影响，或更容易受到高渗透压、氧化应激或高温的影响，其 III 型分泌也未受损。有趣的是，在小鼠全身感染模型中，tatC突变体通过口服和腹膜内途径严重减毒，并且在淋巴器官（如派尔氏淋巴集结和脾脏）的定植中受到严重损害。我们的工作强调，Tat 分泌在假结核杆菌的毒力中起着关键作用。
Yersinia species pathogenic to humans have been extensively characterized with respect to type III secretion and its essential role in virulence. This study concerns the twin arginine translocation (Tat) pathway utilized by gram-negative bacteria to secrete folded proteins across the bacterial inner membrane into the periplasmic compartment. We have shown that the Yersinia Tat system is functional and required for motility and contributes to acid resistance. A Yersinia pseudotuberculosis mutant strain with a disrupted Tat system (tatC) was, however, not affected in in vitro growth or more susceptible to high osmolarity, oxidative stress, or high temperature, nor was it impaired in type III secretion. Interestingly, the tatC mutant was severely attenuated via both the oral and intraperitoneal routes in the systemic mouse infection model and highly impaired in colonization of lymphoid organs like Peyer's patches and the spleen. Our work highlights that Tat secretion plays a key role in the virulence of Y. pseudotuberculosis.