In vivo intracellular recording and perturbation of persistent activity in a neural integrator
In vivo intracellular recording and perturbation of persistent activity in a neural integrator
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DOI：
10.1038/84023
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发表时间：
2001-02-01
影响因子：
25
通讯作者：
Tank, DW
中科院分区：
文献类型：
--
作者：
Aksay, E;Gamkrelidze, G;Tank, DW
为了研究持续性神经活动的机制，我们获得了在自发扫视和固定过程中的金鱼眼神经整合器的神经元在体内的细胞内记录。扫视后放电率的持续变化与棘突间膜电位的阶跃变化相关，而棘突间膜电位与眼位的变化相关。短暂的细胞内电流脉冲，旨在模仿扫视输入的持续性活动的扰动只引起短暂的变化的放电率和膜电位。当神经元超极化低于动作电位阈值时，位置相关的膜电位阶跃变化仍然存在。膜电位波动较大，在更多的去极化步骤。这些结果表明，支持持续的变化，放电率不是由膜多稳态或起搏器电流的变化，而是由突触输入的速率或幅度的持续变化。
To investigate the mechanisms of persistent neural activity, we obtained in vivo intracellular recordings from neurons in an oculomotor neural integrator of the goldfish during spontaneous saccades and fixations. Persistent changes in firing rate following saccades were associated with step changes in interspike membrane potential that were correlated with changes in eye position. Perturbation of persistent activity with brief intracellular current pulses designed to mimic saccadic input only induced transient changes of firing rate and membrane potential. When neurons were hyperpolarized below action potential threshold, position-correlated step changes in membrane potential remained. Membrane potential fluctuations were greater during more depolarized steps. These results suggest that sustained changes in firing rate are supported not by either membrane multistability or changes in pacemaker currents, but rather by persistent changes in the rate or amplitude of synaptic inputs.